Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5217
Title: Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4+ T Cells into IFN gamma IL10 Producing Tbet+ Th1 Cells
Authors: Saygılı, Tahsin
Akıncılar, Semih Can
Akgül, Bünyamin
Nalbant Aldanmaz, Ayten
Saygılı, Tahsin
Akıncılar, Semih Can
Akgül, Bünyamin
Nalbant Aldanmaz, Ayten
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: CD4 antigen
Aggregatibacter actinomycetemcomitans
Tumor necrosis factor alpha
lymphocyte differentiation
Th1 cell
Negibacteria
Issue Date: Nov-2012
Publisher: Public Library of Science
Source: Saygılı, T., Akıncılar, S. C. and Nalbant Aldanmaz, A. (2012). Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4+ T Cells into IFNγ IL10 Producing Tbet+ Th1 Cells. PLoS ONE, 7(11). doi:10.1371/journal.pone.0049252
Abstract: One of the heat shock family protein (Hsp) expressing bacteria is the gram negative, periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa). A. actinomycetemcomitans' Hsp is a 64-kDa GroEL-protein, which has been shown to influence the host cells. In this study we used recombinant A. actinomycetemcomitans GroEL (rAaGroEL) protein as a model antigen to study GroEL-mediated T cell immune response. Human peripheral mononuclear cells (PBMCs), when stimulated with recombinant rAaGroEL, expressed early activation marker CD69 and IL-2R (CD25). CD25 and CD69 expressions were higher in CD4+ T cells compared to CD8+ T cells. rAaGroEL-responding CD4+ T cells expressed IL-10, IFNγ and TNFα cytokines. Interestingly, there were also IL-10 and IFNγ double cytokine producing CD4+ T cells. Additionally, IFNγ expressing CD4+ T cells were also T-bet positive. Altogether the results suggest that rAaGroEL protein affects CD4+ T cells to differentiate into IFNγ IL10-secreting T-bet+ Th1 cells.
URI: http://dx.doi.org/10.1371/journal.pone.0049252
http://hdl.handle.net/11147/5217
ISSN: 1932-6203
1932-6203
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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