Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5064
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dc.contributor.authorAtik, Ahmet Emin-
dc.contributor.authorBozdağ, Gönensin Ozan-
dc.contributor.authorAkıncı, Ersin-
dc.contributor.authorKaya, Alaattin-
dc.contributor.authorKoç, Ahmet-
dc.contributor.authorYalçın, Talat-
dc.contributor.authorKarakaya, Hüseyin Çağlar-
dc.date.accessioned2017-03-15T12:13:54Z
dc.date.available2017-03-15T12:13:54Z
dc.date.issued2011
dc.identifier.citationAtik, A.E., Bozdaǧ, G.O., Akıncı, E., Kaya, A., Koç, A., Yalçın, T., and Karakaya, H. Ç. (2011). Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. Turkish Journal of Botany, 35(4), 379-388. doi:10.3906/bot-1007-29en_US
dc.identifier.issn1300-008X
dc.identifier.issn1300-008X-
dc.identifier.issn1303-6106-
dc.identifier.urihttps://doi.org/10.3906/bot-1007-29
dc.identifier.urihttp://hdl.handle.net/11147/5064
dc.description.abstractBoron is an essential micronutrient for plants and animals; however, it can be toxic when present at high concentrations. The purpose of this study was to understand the mechanisms of boron tolerance in the Turkish barley (Hordeum vulgare) Anadolu cultivar. For this purpose, 2-dimensional electrophoresis (2-DE) was used to screen differentially expressed proteins for both control and boron-stressed Anadolu barley genotypes. Seven proteins were revealed by 2-DE: 1) ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCo large chain), 2) TLP5, a thaumatin-like protein, 3) PR5, a basic pathogenesis-related protein, 4) a RNase S-like protein, 5) a PSI type III chlorophyll a/b-binding protein, 6) a light-harvesting complex I LHC I, and 7) the vacuolar proton-translocating ATPase subunit E protein. These were found to be upregulated in response to boron treatment. Even though the protein encoded by the V-ATPase subunit E gene was overexpressed, its transcript level was downregulated by boron treatment. Heterologous expression of the barley V-ATPase subunit E gene in yeast provided boron resistance to yeast cells. These results indicated that the V-ATPase subunit E gene was functional and conferred tolerance to toxic boron levels in yeast and might play a role in the overall boron tolerance of barley. © TÜBITAK.en_US
dc.description.abstractBor, bitkiler ve hayvanlar için gerekli olan mikrolementlerdendir. Ancak yüksek konsantrasyonlarda toksik etki göstermektedir. Bu çalışmada bora dayanıklı Anadolu arpa (Hordeum vulgare) genotipindeki bor tolerans mekanizmasının anlaşılması amaçlanmıştır. Kontrol ve bor stresi uygulanmış ve Anadolu genotipi arasındaki farklı seviyede ekspres olan proteinleri taramak için 2 boyutlu elektroforez tekniği uygulanmıştır. İki boyutlu elektroforez deneyi sonucu ekspresyon seviyesi artan yedi protein bulunmuştur: ribuloz 1,5-bifosfat karboksilaz/oksijenaz büyük zincir, thaumatin benzeri protein TLP5, patojenaz benzeri protein PR5, RNaz S benzeri protein, PSI tipi III a/bbağlayıcı protein, ışık toplayan kompleks protein I LHC I ve vakuol proton translokasyon ATPaz alt birim E. V-ATPaz alt birim E geni tarafından kodlanan proteinin seviyesinin bor uygulaması ile artmasına rağmen, genin transkript seviyesi azalmıştır. Arpa V-ATPaz alt birim E geninin maya hücrelerinde ifadenlenmesi maya hücrelerine bora karşı dayanıklılık sağlamıştır. Bu sonuçlar, V-ATPaz alt birim E geninin mayada fonksiyonel olduğunu ve maya hücrelerine, toksik bor seviyelerine karşı dayanıklılık sağladığını ve de arpada bor toleransında bir rolü olabileceğini göstermektedir.en_US
dc.description.sponsorshipTUBITAK 104T213; TUBITAK TBAG-2354; IYTE-BAP20en_US
dc.language.isoengen_US
dc.publisherTürkiye Klinikleri Journal of Medical Sciencesen_US
dc.relation.ispartofDoga, Turkish Journal of Botanyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBoron stressen_US
dc.subjectHordeum vulgareen_US
dc.subjectProteomicsen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectV-ATPaseen_US
dc.titleProteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit Een_US
dc.title.alternativeBoron toleransı sırasında arpada (Hordeum vulgare) görülen proteomik değişimler ve vakuol proton translokasyon ATPaz alt birim E’nin rolüen_US
dc.typearticleen_US
dc.authoridTR110769en_US
dc.authoridTR130617en_US
dc.authoridTR24898en_US
dc.contributor.institutionauthorAtik, Ahmet Emin-
dc.contributor.institutionauthorBozdağ, Gönensin Ozan-
dc.contributor.institutionauthorAkıncı, Ersin-
dc.contributor.institutionauthorKaya, Alaattin-
dc.contributor.institutionauthorKoç, Ahmet-
dc.contributor.institutionauthorYalçın, Talat-
dc.contributor.institutionauthorKarakaya, Hüseyin Çağlar-
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume35en_US
dc.identifier.issue4en_US
dc.identifier.startpage379en_US
dc.identifier.endpage388en_US
dc.identifier.wosWOS:000292901400008
dc.identifier.scopusSCOPUS:2-s2.0-79957968766
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.3906/bot-1007-29-
dc.relation.doi10.3906/bot-1007-29en_US
dc.coverage.doi10.3906/bot-1007-29en_US
item.openairetypearticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.deptDepartment of Chemistry-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
Appears in Collections:Chemistry / Kimya
Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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