Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4725
Title: Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli
Authors: Yavuz, Elif
Güneş, Hatice
Harsa, Hayriye Şebnem
Bulut, Çisem
Yenidünya, Ali Fazıl
Keywords: DNA degradation
Genotyping
Pulsed field gel electrophoresis
Bacteriology
Thermophilic bacilli
Issue Date: Nov-2004
Publisher: Springer Verlag
Source: Yavuz, E., Güneş, H., Harsa, H. Ş., Bulut, Ç., and Yenidünya, A. F. (2004). Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli. World Journal of Microbiology and Biotechnology, 20(8), 871-874. doi:10.1007/s11274-004-1004-3
Abstract: Although thermophilic members of the genus Bacillus are known to tolerate extreme environmental conditions, they appeared to be readily damaged upon mechanical manipulations. This fact was evidenced in genotyping of some strains of thermophilic Bacillus by pulsed field gel electrophoresis (PFGE). Consequently, a new procedure for the preparation of agarose plugs was developed. The procedure produced interpretable genomic DNA restriction profiles.
URI: http://doi.org/10.1007/s11274-004-1004-3
http://hdl.handle.net/11147/4725
ISSN: 0959-3993
0959-3993
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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