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https://hdl.handle.net/11147/4725| Title: | Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli | Authors: | Yavuz, Elif Güneş, Hatice Harsa, Hayriye Şebnem Bulut, Çisem Yenidünya, Ali Fazıl |
Keywords: | DNA degradation Genotyping Pulsed field gel electrophoresis Bacteriology Thermophilic bacilli |
Publisher: | Springer Verlag | Source: | Yavuz, E., Güneş, H., Harsa, H. Ş., Bulut, Ç., and Yenidünya, A. F. (2004). Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli. World Journal of Microbiology and Biotechnology, 20(8), 871-874. doi:10.1007/s11274-004-1004-3 | Abstract: | Although thermophilic members of the genus Bacillus are known to tolerate extreme environmental conditions, they appeared to be readily damaged upon mechanical manipulations. This fact was evidenced in genotyping of some strains of thermophilic Bacillus by pulsed field gel electrophoresis (PFGE). Consequently, a new procedure for the preparation of agarose plugs was developed. The procedure produced interpretable genomic DNA restriction profiles. | URI: | http://doi.org/10.1007/s11274-004-1004-3 http://hdl.handle.net/11147/4725 |
ISSN: | 0959-3993 0959-3993 |
| Appears in Collections: | Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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