Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4635
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dc.contributor.authorAkgül, Bünyamin-
dc.contributor.authorTu, Chen-Pei D.-
dc.date.accessioned2016-05-12T11:18:10Z
dc.date.available2016-05-12T11:18:10Z
dc.date.issued2002-09
dc.identifier.citationAkgül, B., and Tu, C.-P. D. (2002). Evidence for a stabilizer element in the untranslated regions of Drosophila glutathione S-transferase D1 mRNA. Journal of Biological Chemistry, 277(38), 34700-34707. doi:10.1074/jbc.M200985200en_US
dc.identifier.issn0021-9258
dc.identifier.issn0021-9258-
dc.identifier.urihttp://doi.org/10.1074/jbc.M200985200
dc.identifier.urihttp://hdl.handle.net/11147/4635
dc.description.abstractThe neighboring genes gstD1 and gstD21 share 70% sequence identity, gstD1 encodes a 1,1,1-trichloro-2,2-bis-(P-chlorophenyl)ethane dehydrochlorinase; gstD21, a ligandin. Both of their mRNAs are inducible by pentobarbital but otherwise behave very differently. Intact gstD21 mRNA is intrinsically labile, but becomes stabilized when separated from its native untranslated region (UTR). In contrast, whereas gstD1 mRNA is very stable in its entirety, without its native UTRs it becomes even more labile than that of gstD21. Decay patterns from four chimeric D1-D21 mRNAs, designed to reveal the individual importance of each molecular region to stability, strongly indicate the presence of destabilizing elements in the coding region ofgstD1 mRNA. Thus, the UTRs of this molecule must contain a dominant stabilizer element that overrides the destabilizing influence of the coding region and confers overall stability to the entire molecule. The suspected presence of such a stabilizer element in gstD1 mRNA extends a concept from mRNA metabolism in yeast and cultured mammalian cells to include a multicellular organism, Drosophila melanogaster. The complementary presence of destabilizing and stabilizer elements on the same mRNA reveals a regulatory mechanism by which an abundant mRNA can be further induced by a chemical stimulus, or otherwise be returned to normal levels during recovery.en_US
dc.language.isoenen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology Inc.en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiochemistryen_US
dc.subjectEnzymesen_US
dc.subjectRNAen_US
dc.subjectYeasten_US
dc.subjectCoding regionsen_US
dc.subjectDrosophila melanogasteren_US
dc.titleEvidence for a stabilizer element in the untranslated regions of Drosophila glutathione S-transferase D1 mRNAen_US
dc.typeArticleen_US
dc.authoridTR37475en_US
dc.institutionauthorAkgül, Bünyamin-
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume277en_US
dc.identifier.issue38en_US
dc.identifier.startpage34700en_US
dc.identifier.endpage34707en_US
dc.identifier.wosWOS:000178117000008en_US
dc.identifier.scopus2-s2.0-0037144503en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1074/jbc.M200985200-
dc.identifier.pmid12118001en_US
dc.relation.doi10.1074/jbc.M200985200en_US
dc.coverage.doi10.1074/jbc.M200985200en_US
dc.identifier.scopusqualityQ1-
item.fulltextWith Fulltext-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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