Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4590
Title: Chromatographic separation of amyloglucosidase from the mixtures of enzymes
Authors: Harsa, Hayriye Şebnem
Furusaki, Shintaro
Harsa, Hayriye Şebnem
Izmir Institute of Technology. Food Engineering
Keywords: Affinity
Bioseparations
Chromatography
Glucoamylase
Beta cyclodextrin
Issue Date: Oct-2001
Publisher: Elsevier Ltd.
Source: Harsa, H. Ş., and Furusaki, S. (2001). Chromatographic separation of amyloglucosidase from the mixtures of enzymes. Biochemical Engineering Journal, 8(3), 257-261. doi:10.1016/S1369-703X(01)00123-1
Abstract: An affinity chromatographic separation of amyloglucosidase (AMG) enzyme using β-cyclodextrin-chitosan matrix was investigated in this study. The adsorbent selectively adsorbed AMG from the mixtures of enzymes (lactase and α-amylase), and this selectively bound AMG was eluted from the column in two steps with a recovery yield of about 72%. A small fraction of AMG and other contaminating proteins did not bind to the column specifically and were eluted easily. Purified AMG was visualised on the sodiumdodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) experiments
URI: http://doi.org/doi:10.1016/S1369-703X(01)00123-1
http://hdl.handle.net/11147/4590
ISSN: 1369-703X
1369-703X
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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