Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4493
Title: Prolactin receptor gene expression in rat splenocytes and thymocytes from birth to adulthood
Authors: Güneş, Hatice
Mastro, Andrea M.
Güneş, Hatice
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Milk
Neonatal immunity
Prolactin
Prolactin receptor
Splenocytes
Thymocytes
Issue Date: Mar-1996
Publisher: Elsevier Ltd.
Source: Güneş, H., and Mastro, A. M. (1996). Prolactin receptor gene expression in rat splenocytes and thymocytes from birth to adulthood. Molecular and Cellular Endocrinology, 117(1), 41-52. doi:10.1016/0303-7207(95)03724-1
Abstract: In vivo and in vitro studies have indicated that the anterior pituitary hormone prolactin (PRL) is an immunoregulator and functions in the development of the neonatal immune system. In this study, prolactin receptor (PRL-R) expression from birth to adulthood as well as the effect of milk ingestion on the PRL-R expression were examined in splenocytes and thymocytes of neonatal rats. Three approaches were taken to measure PRL-R expression: (i) polymerase chain reaction (RT-PCR); (ii) antibody to PRL-R and Western blotting; (iii) antibody to PRL-R and flow cytometry. RT-PCR analysis revealed the short and long form of PRL-R mRNA in both spleen and thymus at every age tested. However, the long form of PRL-R mRNA was always more abundant than that of the short form. In addition, antipeptide antibody against the long form of PRL-R recognized 84 and 42 kD proteins in the spleen, but only the 84 kD protein in the thymus. A monoclonal antibody U6 recognized 38 and 40 kD proteins in both the spleen and thymus. Although the mRNA level of PRL-R was relatively low at birth and increased with age in both the spleen and thymus, the levels of protein bands detected with both antibodies correlated with development in the spleen; whereas the levels remained steady in the thymus. Therefore, we concluded that the expression of PRL-R at the protein level is developmentally regulated in the spleen but not in the thymus. Finally, milk ingestion in the first seven hours decreased the percentage of cells expressing cell surface PRL-R, suggesting that milk-borne PRL may have a direct effect on lymphocytes.
URI: http://doi.org/10.1016/0303-7207(95)03724-1
http://hdl.handle.net/11147/4493
ISSN: 0303-7207
1872-8057
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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