Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2809
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dc.contributor.authorÇakır, Zeynep-
dc.contributor.authorSaydam, Güray-
dc.contributor.authorŞahin, Fahri-
dc.contributor.authorBaran, Yusuf-
dc.date.accessioned2017-01-18T08:03:26Z
dc.date.available2017-01-18T08:03:26Z
dc.date.issued2011-02
dc.identifier.citationÇakır, Z., Saydam, G., Şahin, F., and Baran, Y. (2011). The roles of bioactive sphingolipids in resveratrol-induced apoptosis in HL60 acute myeloid leukemia cells. Journal of Cancer Research and Clinical Oncology, 137 (2), 279-286. doi:10.1007/s00432-010-0884-xen_US
dc.identifier.issn0171-5216
dc.identifier.issn0171-5216-
dc.identifier.issn1432-1335-
dc.identifier.urihttp://doi.org/10.1007/s00432-010-0884-x
dc.identifier.urihttp://hdl.handle.net/11147/2809
dc.description.abstractPurpose Acute promyelocytic leukemia results from a translocation between 15 and 17 chromosomes that produce PML/RARa fusion protein. PML/RARa inhibits differentiation of myeloid precursor cells at stem cell level. Resveratrol is a phytoalexin that exerts cytotoxic effects on cancer cells. Ceramides have crucial roles in cell growth, proliferation, differentiation, drug resistance, and apoptosis. In this study, we examined the possible cytotoxic effects of resveratrol on acute myeloid leukemia cells and determined the roles of ceramide-metabolizing genes in resveratrol-induced apoptosis, in addition to investigating the possibility of increasing the sensitivity of HL60 cells to resveratrol by manipulating sphingolipids. Methods Cytotoxic effects of resveratrol, C8:ceramide, PDMP, and SK-1 inhibitor were determined by XTT cell proliferation assay. Changes in caspase-3 enzyme activity and mitochondrial membrane potential (MMP) were measured using caspase-3 colorimetric assay and JC-1 MMP detection kit. Expression levels of ceramide-metabolizing genes were examined by RT-PCR. Results The results revealed that manipulations of ceramide metabolism toward generation or accumulation of apoptotic ceramides increased apoptotic effects of resveratrol in HL60 cells, synergistically. More importantly, gene expression analyses revealed that resveratrol-induced apoptosis via increasing expression levels of ceramide generating genes and decreasing expression levels of antiapoptotic sphingosine kinase-1 and glucosylceramide synthase genes. Conclusion These results showed for the first time that increasing intracellular levels of ceramides by biochemical approaches has also increased sensitivity of HL60 cells to resveratrol. We also showed that resveratrol induces apoptosis through manipulating ceramide-metabolizing genes that resulted in the accumulation of ceramides in HL60 cells.en_US
dc.description.sponsorshipThe Turkish Society of Hematologyen_US
dc.language.isotren_US
dc.publisherSpringer Verlagen_US
dc.relation.ispartofJournal of Cancer Research and Clinical Oncologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCeramidesen_US
dc.subjectBioactive sphingolipidsen_US
dc.subjectResveratrolen_US
dc.subjectAcute myeloid leukemiaen_US
dc.subjectHL60en_US
dc.titleThe roles of bioactive sphingolipids in resveratrol-induced apoptosis in HL60 acute myeloid leukemia cellsen_US
dc.typeArticleen_US
dc.authoridTR119193en_US
dc.institutionauthorÇakır, Zeynep-
dc.institutionauthorBaran, Yusuf-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume137en_US
dc.identifier.issue2en_US
dc.identifier.startpage279en_US
dc.identifier.endpage286en_US
dc.identifier.wosWOS:000286106000012en_US
dc.identifier.scopus2-s2.0-78751643035en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s00432-010-0884-x-
dc.identifier.pmid20401667en_US
dc.relation.doi10.1007/s00432-010-0884-xen_US
dc.coverage.doi10.1007/s00432-010-0884-xen_US
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.languageiso639-1tr-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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