Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2672
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dc.contributor.authorKanbar, Bora-
dc.contributor.authorÖzdemir, Ekrem-
dc.date.accessioned2016-12-26T07:36:07Z
dc.date.available2016-12-26T07:36:07Z
dc.date.issued2010-09
dc.identifier.citationKanbar, B., and Özdemir, E. (2010). Thermal stability of carbonic anhydrase immobilized within polyurethane foam. Biotechnology Progress, 26(5), 1474-1480. doi:10.1002/btpr.452en_US
dc.identifier.issn1520-6033
dc.identifier.issn1520-6033-
dc.identifier.urihttp://doi.org/10.1002/btpr.452
dc.identifier.urihttp://hdl.handle.net/11147/2672
dc.description.abstractThermal stability of carbonic anhydrase (CA) immobilized within polyurethane (PU) foam was investigated. The catalytic activity of the enzyme was estimated by using p-nitrophenyl acetate (p-NPA) as the substrate in tris buffer containing 10% acetonitrile. The immobilized CA was stable during the repeatable washings and stability tests over 45 days stored in tris buffer at ambient conditions indicating that the CA was covalently attached to the polyurethane (PU) foam by crosslinking. The immobilized CA was found to be 98% stable below 50°C, whereas a drastic decrease was seen at temperatures between 50 and 60°C. The optimum temperature for the immobilized CA was found to be 45°C and it lost its activity completely at 60°C. Thermal deactivation energies for the free and immobilized CA were estimated to be 29 and 86 kcal/mol, respectively. The association of unfolded CA with the polymeric backbone chains of the PU foam was also addressed. It was concluded that the immobilized CA was highly stable at temperatures less than 50°C and could be used in biomimetic CO sequestration processes. © 2010 American Institute of Chemical Engineersen_US
dc.description.sponsorshipIzmir Institute of Technology project numbers 2006IYTE29 and 2008IYTE15; Dow Chemicals HYPOL2060en_US
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Inc.en_US
dc.relation.ispartofBiotechnology Progressen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCarbonic anhydraseen_US
dc.subjectCO2 sequestrationen_US
dc.subjectThermal stabilityen_US
dc.subjectEnzymesen_US
dc.subjectPolyurethane foamen_US
dc.subjectImmobilizationen_US
dc.titleThermal stability of carbonic anhydrase immobilized within polyurethane foamen_US
dc.typeArticleen_US
dc.authoridTR114257en_US
dc.institutionauthorKanbar, Bora-
dc.institutionauthorÖzdemir, Ekrem-
dc.departmentİzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume26en_US
dc.identifier.issue5en_US
dc.identifier.startpage1474en_US
dc.identifier.endpage1480en_US
dc.identifier.wosWOS:000283482100032en_US
dc.identifier.scopus2-s2.0-79952279710en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1002/btpr.452-
dc.identifier.pmid20945495en_US
dc.relation.doi10.1002/btpr.452en_US
dc.coverage.doi10.1002/btpr.452en_US
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ2-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.dept03.02. Department of Chemical Engineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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