Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2485
Title: Extracellular enzyme production and enterotoxigenic gene profiles of Bacillus cereus and Bacillus thuringiensis strains isolated from cheese in Turkey
Authors: Molva, Çelenk
Sudağıdan, Mert
Okuklu, Burcu
Molva, Çelenk
Sudağıdan, Mert
Okuklu, Burcu
Izmir Institute of Technology. Food Engineering
Izmir Institute of Technology. Biotechnology and Bioengineering Research and Application Center (IZTECH BIYOMER)
Keywords: Bacillus cereus
Bacillus thuringiensis
Cheese
Enterotoxin genes
Issue Date: Sep-2009
Publisher: Elsevier Ltd.
Source: Molva, Ç., Sudağıdan, M., and Okuklu, B. (2009). Extracellular enzyme production and enterotoxigenic gene profiles of Bacillus cereus and Bacillus thuringiensis strains isolated from cheese in Turkey. Food Control, 20(9), 829-834. doi:10.1016/j.foodcont.2008.10.016
Abstract: The aim of the present study was to investigate the biochemical characteristics, extracellular enzyme production and enterotoxigenic genes contents of 6 Bacillus cereus and 22 Bacillus thuringiensis strains, isolated from 100 cheese samples in Turkey. Crystal morphologies of B. thuringiensis strains were found either spherical (n = 12) or spherical and irregular-shaped (n = 10) by phase contrast microscopy. B. cereus and B. thuringiensis strains were found to produce extracellular enzymes, respectively: gelatinase (83% and 91%), DNase (83% and 77%), lecithinase (83% and 95%), protease on skim milk agar (100% and 100%), protease on milk agar (100% and 91%), protease on casein agar (83% and 77%), xylanase (100% and 45%), and cellulase (0% and 41%), and amylase (83% and 27%). All of the strains, except for Bt-D1, hydrolyzed Tween 20 (96%), but not Tween 80 or tributyrin. Pectinolytic activity was obtained to be the least frequent (4%). PCR analysis showed that all strains contained nheA, nheB, nheC and hblD genes. The hblA and hblC genes were present in 2 and 4 of B. thuringiensis strains, respectively. The bceT gene was detected in 1 B. cereus and 9 B. thuringiensis strains. The entFM gene was detected more frequently in B. thuringiensis (82%) than in B. cereus strains (50%). To our knowledge, this is the first report about the isolation and identification of enterotoxigenic B. cereus and B. thuringiensis strains from cheese samples in Turkey.
URI: http://dx.doi.org/10.1016/j.foodcont.2008.10.016
http://hdl.handle.net/11147/2485
ISSN: 0956-7135
0956-7135
Appears in Collections:Food Engineering / Gıda Mühendisliği
IZTECH Research Centers Collection / İYTE Araştırma Merkezleri Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
2485.pdfMakale402.47 kBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

SCOPUSTM   
Citations

36
checked on Sep 18, 2021

WEB OF SCIENCETM
Citations

37
checked on Sep 18, 2021

Page view(s)

16
checked on Sep 20, 2021

Download(s)

24
checked on Sep 20, 2021

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.