Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2485
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dc.contributor.authorMolva, Çelenk-
dc.contributor.authorSudağıdan, Mert-
dc.contributor.authorOkuklu, Burcu-
dc.date.accessioned2016-11-21T13:45:17Z
dc.date.available2016-11-21T13:45:17Z
dc.date.issued2009-09
dc.identifier.citationMolva, Ç., Sudağıdan, M., and Okuklu, B. (2009). Extracellular enzyme production and enterotoxigenic gene profiles of Bacillus cereus and Bacillus thuringiensis strains isolated from cheese in Turkey. Food Control, 20(9), 829-834. doi:10.1016/j.foodcont.2008.10.016en_US
dc.identifier.issn0956-7135
dc.identifier.issn0956-7135-
dc.identifier.urihttp://dx.doi.org/10.1016/j.foodcont.2008.10.016
dc.identifier.urihttp://hdl.handle.net/11147/2485
dc.description.abstractThe aim of the present study was to investigate the biochemical characteristics, extracellular enzyme production and enterotoxigenic genes contents of 6 Bacillus cereus and 22 Bacillus thuringiensis strains, isolated from 100 cheese samples in Turkey. Crystal morphologies of B. thuringiensis strains were found either spherical (n = 12) or spherical and irregular-shaped (n = 10) by phase contrast microscopy. B. cereus and B. thuringiensis strains were found to produce extracellular enzymes, respectively: gelatinase (83% and 91%), DNase (83% and 77%), lecithinase (83% and 95%), protease on skim milk agar (100% and 100%), protease on milk agar (100% and 91%), protease on casein agar (83% and 77%), xylanase (100% and 45%), and cellulase (0% and 41%), and amylase (83% and 27%). All of the strains, except for Bt-D1, hydrolyzed Tween 20 (96%), but not Tween 80 or tributyrin. Pectinolytic activity was obtained to be the least frequent (4%). PCR analysis showed that all strains contained nheA, nheB, nheC and hblD genes. The hblA and hblC genes were present in 2 and 4 of B. thuringiensis strains, respectively. The bceT gene was detected in 1 B. cereus and 9 B. thuringiensis strains. The entFM gene was detected more frequently in B. thuringiensis (82%) than in B. cereus strains (50%). To our knowledge, this is the first report about the isolation and identification of enterotoxigenic B. cereus and B. thuringiensis strains from cheese samples in Turkey.en_US
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.relation.ispartofFood Controlen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacillus cereusen_US
dc.subjectBacillus thuringiensisen_US
dc.subjectCheeseen_US
dc.subjectEnterotoxin genesen_US
dc.titleExtracellular enzyme production and enterotoxigenic gene profiles of Bacillus cereus and Bacillus thuringiensis strains isolated from cheese in Turkeyen_US
dc.typeArticleen_US
dc.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.departmentIzmir Institute of Technology. Biotechnology and Bioengineering Research and Application Center (IZTECH BIYOMER)en_US
dc.identifier.volume20en_US
dc.identifier.issue9en_US
dc.identifier.startpage829en_US
dc.identifier.endpage834en_US
dc.identifier.wosWOS:000265906400011
dc.identifier.scopusSCOPUS:2-s2.0-63449097784
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.foodcont.2008.10.016-
dc.relation.doi10.1016/j.foodcont.2008.10.016en_US
dc.coverage.doi10.1016/j.foodcont.2008.10.016en_US
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
Appears in Collections:Food Engineering / Gıda Mühendisliği
IZTECH Research Centers Collection / İYTE Araştırma Merkezleri Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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