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Title: Isolation and characterization of Bacillus thuringiensis strains from olive-related habitats in Turkey
Authors: Çınar, Çelenk
Apaydın, Özgür
Yenidünya, Ali Fazıl
Harsa, Hayriye Şebnem
Güneş, Hatice
Keywords: Bacillus thuringiensis
Crystal protein
Polymerase chain reaction
Issue Date: Feb-2008
Publisher: John Wiley and Sons Inc.
Source: Çınar, Ç., Apaydın, Ö., Yenidünya, A. F., Harsa, H. Ş., and Güneş, H. (2008). Isolation and characterization of Bacillus thuringiensis strains from olive-related habitats in Turkey. Journal of Applied Microbiology, 104(2), 515-525. doi:10.1111/j.1365-2672.2007.03571.x
Abstract: Aims: To isolate Bacillus thuringiensis strains from different olive-related habitats (olive groves and olive oil factories) in Turkey and to characterize these strains by molecular methods. Methods and Results: A total of 150 samples, consisting of olive grove soil, green olive leaves, olive leaf residues, animal faeces, olive pomace and dust, were examined for the presence of B. thuringiensis. One hundred B. thuringiensis strains were isolated from 54 environmental samples (36%) and characterized in terms of crystal morphology, cry and cyt gene content by polymerase chain reaction, plasmid profiles and 16S-internal transcribed spacer ribosomal DNA restriction fragment length polymorphism (16S-ITS rDNA RFLP). The highest percentage of samples containing B. thuringiensis was found in 38 out of 54 total soil samples (70%). Of the 100 B. thuringiensis isolates, the most frequent crystal shapes were irregularly shaped (24%), spherical-irregular pointed (19%), cuboidal (17%) and spherical (16%). The cry1 plus cry4 genotype was the most abundant genotype in our collection (21%). RFLP analysis of the amplified 16S-ITS rDNA revealed 11 distinct patterns for the isolates and 10 reference strains. Conclusions: Bacillus thuringiensis isolates showed a great genetic diversity and crystal shape heterogeneity. Significance and Impact of the Study: This is the first study on the isolation and characterization of B. thuringiensis from olive-related habitats in Turkey. No correlation was observed between the cry genotypes and insecticidal crystal shapes of the isolates. Restriction profiles of 23% of the isolates were found to be different from those of the 10 reference strains used.
ISSN: 1364-5072
Appears in Collections:Food Engineering / Gıda Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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