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Title: Composition of carotenoids in Scenedesmus protuberans: Application of chromatographic and spectroscopic methods
Authors: Erdoğan, Ayşegül
Çağır, Ali
Conk Dalay, Meltem
Eroğlu, Ahmet Emin
Keywords: Pigments
Chromatography and spectroscopy
Method validation
Scenedesmus protuberans
Issue Date: Sep-2015
Publisher: Springer Verlag
Source: Erdoğan, A., Çağır, A., Conk Dalay, M., and Eroğlu, A. E. (2008). Composition of carotenoids in Scenedesmus protuberans: Application of chromatographic and spectroscopic methods. Food Analytical Methods, 8(8), 1970-1978. doi:10.1007/s12161-015-0088-8
Abstract: This study aimed to identify and determine the carotenoids from green microalga, Scenedesmus protuberans using analytical techniques. Identification of carotenoids was realized by comparing their absorption and mass spectral data with those of reference standards available and reported values. Chromatographic data were then combined with the spectroscopic information. The separation of carotenoids was achieved by C30 column and high-performance liquid chromatography-diode array detection was used for their determination. In the present work, the carotenoid content of S. protuberans was found to be 1.45 mg/g of violaxanthin, 2.47 mg/g of all-trans-lutein, 0.15 mg/g of all-trans-α-carotene, 0.55 mg/g of all-trans-β-carotene, and 0.20 mg/g of 9 or 9′-cis-β-carotene. Due to lack of their standards, the amount of all-trans-loroxanthin and cis-isomers of other carotenoids could not be quantified. In order to validate the method, Certified Reference Material (BCR 485-Mixed vegetables) was used. In conclusion, this study can serve as a reference for the analysis of carotenoids in other microalgae.
ISSN: 1936-9751
Appears in Collections:Chemistry / Kimya
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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