Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2307
Title: Biomimetic CO2 sequestration: 1. Immobilization of carbonic anhydrase within polyurethane foam
Authors: Özdemir, Ekrem
Keywords: Carbonic anhydrase
Ambient conditions
Tris buffers
Catalytic activity
Aqueous phase
Issue Date: Nov-2009
Publisher: American Chemical Society
Source: Özdemir, E. (2009). Biomimetic CO2 sequestration: 1. Immobilization of carbonic anhydrase within polyurethane foam. Energy and Fuels, 23(11), 5725-5730. doi:10.1021/ef9005725
Abstract: Bovine carbonic anhydrase (CA) was immobilized within polyurethane (PU) foam for biomimetic CO2 sequestration. The catalytic activities for the free and immobilized CA were estimated using paranitrophenyl acetate (p-NPA) as the substrate. Because the p-NPA has limited solubility in the aqueous phase, the activities were estimated in Tris buffer containing 10% acetonitrile. A Lineweaver-Burk relationship was employed to estimate the Michaelis-Menten kinetic parameters for the free and immobilized CA. The kcat, K m, and kcat/Km values for the free CA were found to be 2.02 s-1, 12.2 mM, and 166.4M-1 s-1, respectively. The Km value for the immobilized CA was estimated to be 9.6mMat the same conditions. The immobilized CA was stable and did not lose any activity over seven consecutive washings and activity tests. While the free CA lost its activity in 45 days stored at 4 °C in refrigerator, the immobilized CA maintained 100% of its activity over a 45 day period stored in Tris buffer at ambient conditions. It was concluded that the immobilized CA as a very stable biocatalyst could be employed in biomimetic CO2 sequestration.
URI: http://dx.doi.org/10.1021/ef9005725
http://hdl.handle.net/11147/2307
ISSN: 0887-0624
0887-0624
1520-5029
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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