Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2307
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dc.contributor.authorÖzdemir, Ekrem-
dc.date.accessioned2016-10-21T12:41:36Z
dc.date.available2016-10-21T12:41:36Z
dc.date.issued2009-11
dc.identifier.citationÖzdemir, E. (2009). Biomimetic CO2 sequestration: 1. Immobilization of carbonic anhydrase within polyurethane foam. Energy and Fuels, 23(11), 5725-5730. doi:10.1021/ef9005725en_US
dc.identifier.issn0887-0624
dc.identifier.issn0887-0624-
dc.identifier.issn1520-5029-
dc.identifier.urihttp://dx.doi.org/10.1021/ef9005725
dc.identifier.urihttp://hdl.handle.net/11147/2307
dc.description.abstractBovine carbonic anhydrase (CA) was immobilized within polyurethane (PU) foam for biomimetic CO2 sequestration. The catalytic activities for the free and immobilized CA were estimated using paranitrophenyl acetate (p-NPA) as the substrate. Because the p-NPA has limited solubility in the aqueous phase, the activities were estimated in Tris buffer containing 10% acetonitrile. A Lineweaver-Burk relationship was employed to estimate the Michaelis-Menten kinetic parameters for the free and immobilized CA. The kcat, K m, and kcat/Km values for the free CA were found to be 2.02 s-1, 12.2 mM, and 166.4M-1 s-1, respectively. The Km value for the immobilized CA was estimated to be 9.6mMat the same conditions. The immobilized CA was stable and did not lose any activity over seven consecutive washings and activity tests. While the free CA lost its activity in 45 days stored at 4 °C in refrigerator, the immobilized CA maintained 100% of its activity over a 45 day period stored in Tris buffer at ambient conditions. It was concluded that the immobilized CA as a very stable biocatalyst could be employed in biomimetic CO2 sequestration.en_US
dc.description.sponsorshipBAP2006-IYTE-29en_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofEnergy and Fuelsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCarbonic anhydraseen_US
dc.subjectAmbient conditionsen_US
dc.subjectTris buffersen_US
dc.subjectCatalytic activityen_US
dc.subjectAqueous phaseen_US
dc.titleBiomimetic CO2 sequestration: 1. Immobilization of carbonic anhydrase within polyurethane foamen_US
dc.typeArticleen_US
dc.authoridTR114257en_US
dc.institutionauthorÖzdemir, Ekrem-
dc.departmentİzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume23en_US
dc.identifier.issue11en_US
dc.identifier.startpage5725en_US
dc.identifier.endpage5730en_US
dc.identifier.wosWOS:000272700200051en_US
dc.identifier.scopus2-s2.0-73249127004en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1021/ef9005725-
dc.relation.doi10.1021/ef9005725en_US
dc.coverage.doi10.1021/ef9005725en_US
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
dc.identifier.wosqualityttpTop10%en_US
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.dept03.02. Department of Chemical Engineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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