Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/14256
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dc.contributor.authorAkçaöz Alasar, Azime-
dc.contributor.authorTuncel, Özge-
dc.contributor.authorSağlam, Buket-
dc.contributor.authorGazaloğlu, Yasemin-
dc.contributor.authorAtbinek, Melis-
dc.contributor.authorÇağıral, Umut-
dc.contributor.authorİşcan, Evin-
dc.contributor.authorÖzhan, Güneş-
dc.contributor.authorAkgül, Bünyamin-
dc.date.accessioned2024-01-30T09:24:43Z-
dc.date.available2024-01-30T09:24:43Z-
dc.date.issued2024-
dc.identifier.issn0021-9541-
dc.identifier.issn1097-4652-
dc.identifier.urihttps://doi.org/10.1002/jcp.31176-
dc.identifier.urihttps://hdl.handle.net/11147/14256-
dc.description.abstractTumor necrosis factor-alpha (TNF-alpha) is a ligand that induces both intrinsic and extrinsic apoptotic pathways in HeLa cells by modulating complex gene regulatory mechanisms. However, the full spectrum of TNF-alpha-modulated epitranscriptomic m(6)A marks is unknown. We employed a genomewide approach to examine the extent of m(6)A RNA modifications under TNF-alpha-modulated apoptotic conditions in HeLa cells. miCLIP-seq analyses revealed a plethora of m(6)A marks on 632 target mRNAs with an enrichment on 99 mRNAs associated with apoptosis. Interestingly, the m(6)A RNA modification patterns were quite different under cisplatin- and TNF-alpha-mediated apoptotic conditions. We then examined the abundance and translational efficiencies of several mRNAs under METTL3 knockdown and/or TNF-alpha treatment conditions. Our analyses showed changes in the translational efficiency of TP53INP1 mRNA based on the polysome profile analyses. Additionally, TP53INP1 protein amount was modulated by METTL3 knockdown upon TNF-alpha treatment but not CP treatment, suggesting the existence of a pathway-specific METTL3-TP53INP1 axis. Congruently, METLL3 knockdown sensitized HeLa cells to TNF-alpha-mediated apoptosis, which was also validated in a zebrafish larval xenograft model. These results suggest that apoptotic pathway-specific m(6)A methylation marks exist in cells and TNF-alpha-METTL3-TP53INP1 axis modulates TNF-alpha-mediated apoptosis in HeLa cells.en_US
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma Kurumuen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Cellular Physiologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosisen_US
dc.subjectEpitranscriptomicsen_US
dc.subjectHeLa cellsen_US
dc.subjectRNA modificationen_US
dc.titleEpitranscriptomics m6A analyses reveal distinct m6A marks under tumor necrosis factor α (TNF-α)-induced apoptotic conditions in HeLa cellsen_US
dc.typeArticleen_US
dc.typeArticle; Early Accessen_US
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.wosWOS:001136732000001en_US
dc.identifier.scopus2-s2.0-85181508748en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1002/jcp.31176-
dc.identifier.pmid38179601en_US
dc.authorscopusid58452318200-
dc.authorscopusid57210852237-
dc.authorscopusid57563351500-
dc.authorscopusid58455147400-
dc.authorscopusid58454431600-
dc.authorscopusid58452318300-
dc.authorscopusid56711929200-
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.fulltextWith Fulltext-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.openairetypeArticle; Early Access-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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