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https://hdl.handle.net/11147/14256
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DC Field | Value | Language |
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dc.contributor.author | Akçaöz Alasar, Azime | - |
dc.contributor.author | Tuncel, Özge | - |
dc.contributor.author | Sağlam, Buket | - |
dc.contributor.author | Gazaloğlu, Yasemin | - |
dc.contributor.author | Atbinek, Melis | - |
dc.contributor.author | Çağıral, Umut | - |
dc.contributor.author | İşcan, Evin | - |
dc.contributor.author | Özhan, Güneş | - |
dc.contributor.author | Akgül, Bünyamin | - |
dc.date.accessioned | 2024-01-30T09:24:43Z | - |
dc.date.available | 2024-01-30T09:24:43Z | - |
dc.date.issued | 2024 | - |
dc.identifier.issn | 0021-9541 | - |
dc.identifier.issn | 1097-4652 | - |
dc.identifier.uri | https://doi.org/10.1002/jcp.31176 | - |
dc.identifier.uri | https://hdl.handle.net/11147/14256 | - |
dc.description.abstract | Tumor necrosis factor-alpha (TNF-alpha) is a ligand that induces both intrinsic and extrinsic apoptotic pathways in HeLa cells by modulating complex gene regulatory mechanisms. However, the full spectrum of TNF-alpha-modulated epitranscriptomic m(6)A marks is unknown. We employed a genomewide approach to examine the extent of m(6)A RNA modifications under TNF-alpha-modulated apoptotic conditions in HeLa cells. miCLIP-seq analyses revealed a plethora of m(6)A marks on 632 target mRNAs with an enrichment on 99 mRNAs associated with apoptosis. Interestingly, the m(6)A RNA modification patterns were quite different under cisplatin- and TNF-alpha-mediated apoptotic conditions. We then examined the abundance and translational efficiencies of several mRNAs under METTL3 knockdown and/or TNF-alpha treatment conditions. Our analyses showed changes in the translational efficiency of TP53INP1 mRNA based on the polysome profile analyses. Additionally, TP53INP1 protein amount was modulated by METTL3 knockdown upon TNF-alpha treatment but not CP treatment, suggesting the existence of a pathway-specific METTL3-TP53INP1 axis. Congruently, METLL3 knockdown sensitized HeLa cells to TNF-alpha-mediated apoptosis, which was also validated in a zebrafish larval xenograft model. These results suggest that apoptotic pathway-specific m(6)A methylation marks exist in cells and TNF-alpha-METTL3-TP53INP1 axis modulates TNF-alpha-mediated apoptosis in HeLa cells. | en_US |
dc.description.sponsorship | Turkiye Bilimsel ve Teknolojik Arastirma Kurumu | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.relation.ispartof | Journal of Cellular Physiology | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Epitranscriptomics | en_US |
dc.subject | HeLa cells | en_US |
dc.subject | RNA modification | en_US |
dc.title | Epitranscriptomics m6A analyses reveal distinct m6A marks under tumor necrosis factor α (TNF-α)-induced apoptotic conditions in HeLa cells | en_US |
dc.type | Article | en_US |
dc.type | Article; Early Access | en_US |
dc.department | İzmir Institute of Technology. Molecular Biology and Genetics | en_US |
dc.identifier.wos | WOS:001136732000001 | en_US |
dc.identifier.scopus | 2-s2.0-85181508748 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1002/jcp.31176 | - |
dc.identifier.pmid | 38179601 | en_US |
dc.authorscopusid | 58452318200 | - |
dc.authorscopusid | 57210852237 | - |
dc.authorscopusid | 57563351500 | - |
dc.authorscopusid | 58455147400 | - |
dc.authorscopusid | 58454431600 | - |
dc.authorscopusid | 58452318300 | - |
dc.authorscopusid | 56711929200 | - |
dc.identifier.wosquality | Q1 | - |
dc.identifier.scopusquality | Q1 | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.cerifentitytype | Publications | - |
item.openairetype | Article | - |
item.openairetype | Article; Early Access | - |
crisitem.author.dept | 04.03. Department of Molecular Biology and Genetics | - |
crisitem.author.dept | 04.03. Department of Molecular Biology and Genetics | - |
crisitem.author.dept | 04.03. Department of Molecular Biology and Genetics | - |
Appears in Collections: | Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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File | Size | Format | |
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Journal Cellular Physiology.pdf | 3.26 MB | Adobe PDF | View/Open |
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