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https://hdl.handle.net/11147/14156
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Önbaş, Rabia | - |
dc.contributor.author | Arslan Yıldız, Ahu | - |
dc.date.accessioned | 2024-01-06T07:21:35Z | - |
dc.date.available | 2024-01-06T07:21:35Z | - |
dc.date.issued | 2023 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://doi.org/10.1089/ten.tea.2023.0197 | - |
dc.identifier.uri | https://hdl.handle.net/11147/14156 | - |
dc.description.abstract | Patterning cells to create three-dimensional (3D) cell culture models by magnetic manipulation is a promising technique, which is rapid, simple, and cost-effective. This study introduces a new biopatterning approach based on magnetic manipulation of cells with a bioink that consists alginate, cells, and magnetic nanoparticles. Plackett-Burman and Box-Behnken experimental design models were used to optimize bioink formulation where NIH-3T3 cells were utilized as a model cell line. The patterning capability was confirmed by light microscopy through 7 days culture time. Then, biopatterned 3D cardiac structures were formed using H9c2 cardiomyocyte cells. Cellular and extracellular components, F-actin and collagen Type I, and cardiac-specific biomarkers, Troponin T and MYH6, of biopatterned 3D cardiac structures were observed successfully. Moreover, Doxorubicin (DOX)-induced cardiotoxicity was investigated for developed 3D model, and IC50 value was calculated as 8.1 μM for biopatterned 3D cardiac structures, which showed higher resistance against DOX-exposure compared to conventional two-dimensional cell culture. Hereby, developed biopatterning methodology proved to be a simple and rapid approach to fabricate 3D cardiac models, especially for drug screening applications. Copyright 2023, Mary Ann Liebert, Inc., publishers. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Mary Ann Liebert, Inc | en_US |
dc.relation | Manyetik Levitasyon Yöntemi ile 3B Kardiyak Doku Modeli Oluşturulması | - |
dc.relation.ispartof | Tissue Engineering - Part A | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | 3D cell culture | en_US |
dc.subject | Cardiac tissue engineering | en_US |
dc.subject | Contactless manipulation | en_US |
dc.subject | DOX-induced cardiotoxicity | en_US |
dc.subject | Magnetic biopatterning | en_US |
dc.title | Biopatterning of 3D cellular model by contactless magnetic manipulation for cardiotoxicity screening | en_US |
dc.type | Article | en_US |
dc.authorid | 0000-0003-0348-0575 | - |
dc.department | İzmir Institute of Technology. Bioengineering | en_US |
dc.identifier.wos | WOS:001124872100001 | en_US |
dc.identifier.scopus | 2-s2.0-85180299972 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1089/ten.tea.2023.0197 | - |
dc.identifier.pmid | 37974427 | en_US |
dc.relation.grantno | 119Z569 | - |
dc.authorscopusid | 57190064834 | - |
dc.authorscopusid | 57217604248 | - |
item.grantfulltext | none | - |
item.openairetype | Article | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
crisitem.author.dept | 03.01. Department of Bioengineering | - |
Appears in Collections: | Bioengineering / Biyomühendislik PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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