Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/14017
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dc.contributor.authorKeçili, Seren-
dc.contributor.authorYılmaz, Esra-
dc.contributor.authorÖzçelik, Özge Solmaz-
dc.contributor.authorAnıl İnevi, Müge-
dc.contributor.authorGünyüz, Zehra Elif-
dc.contributor.authorYalçın Özuysal, Özden-
dc.contributor.authorÖzçivici, Engin-
dc.contributor.authorTekin, Hüseyin Cumhur-
dc.date.accessioned2023-11-11T08:56:15Z-
dc.date.available2023-11-11T08:56:15Z-
dc.date.issued2023-
dc.identifier.issn2590-1370-
dc.identifier.urihttps://doi.org/10.1016/j.biosx.2023.100392-
dc.identifier.urihttps://hdl.handle.net/11147/14017-
dc.description.abstractCirculating tumor cells (CTCs) are crucial indicators of cancer metastasis. However, their rarity in the bloodstream and the heterogeneity of their surface biomarkers present challenges for their isolation. Here, we developed a hybrid microfluidic platform (microfluidic-based density-associated cell sorting (µDACS) platform) that utilizes density as a biophysical marker to sort cancer cells from the population of white blood cells (WBCs). The platform utilizes the magnetic levitation technique on a microfluidic chip to sort cells based on their specific density ranges, operating under a continuous flow condition. By harnessing magnetic, gravitational, and drag forces, the platform efficiently separates cells. This approach involves a microfluidic chip equipped with a microseparator, which directs cells into top and bottom outlets depending on their levitation heights, which are inversely proportional to their densities. Hence, low-density cancer cells are collected from the top outlet, while high-density WBCs are collected from the bottom outlet. We optimized the sorting efficiency by varying the flow rates, and concentrations of the sorting medium's paramagnetic properties using standard densities of polymeric microspheres. To demonstrate the platform's applicability, we performed hybrid microfluidic sorting on MDA-MB-231 human breast cancer cells and U-937 human monocytes. The results showed efficient sorting of rare cancer cells (≥100 cells/mL) from serum samples, achieving a sorting efficiency of ∼70% at a fast-processing speed of 1 mL h−1. This label-free approach holds promise for rapid and cost-effective CTC sorting, facilitating in-vitro diagnosis and prognosis of cancer. © 2023 The Author(s)en_US
dc.description.sponsorshipH.C.T. gratefully acknowledges financial support from The Scientific and Technological Research Council of Türkiye (116M298), Türkiye, Turkish Academy of Science (TUBA-GEBIP 2020), Türkiye, and Science Academy (BAGEP), Türkiye. S.K. acknowledges the support of Turkish Council of Higher Education, Türkiye for 100/2000 CoHE doctoral scholarship. The authors would like to thank Meltem Elitas, Ph.D. from the Department of Mechatronics Engineering, Sabanci University, Türkiye for providing U-937 cell lines and helpful discussions. The authors would like to also thank Research and Application Center for Quantum Technologies (KUANTAM), IZTECH, Türkiye and Lutfi Ozyuzer, Ph.D. from the Department of Physic, IZTECH for providing cleanroom facilities. The authors would like to thank National Nanotechnology Research Center (UNAM), Türkiye for its support for microfabrication. S.K. and O.S.O. would like to thank Oyku Doyran from the Department of Bioengineering, IZTECH for her helpful support during the experiments.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relationKanser Hücrelerinin Işaretleme Yöntemi Kullanılmaksızın Manyetik Levitasyon Prensibi Ile Mikroakışkan Çip Üzerinde Ayrıştırılması-
dc.relation.ispartofBiosensors and Bioelectronics: Xen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiophysical biomarkersen_US
dc.subjectCirculating tumor cellsen_US
dc.subjectDensityen_US
dc.subjectHybrid cell sortingen_US
dc.subjectLabel-freeen_US
dc.subjectMagnetic levitationen_US
dc.titleμDACS platform: A hybrid microfluidic platform using magnetic levitation technique and integrating magnetic, gravitational, and drag forces for density-based rare cancer cell sortingen_US
dc.typeArticleen_US
dc.departmentİzmir Institute of Technology. Bioengineeringen_US
dc.departmentİzmir Institute of Technology. Molecular Biology and Genetics-
dc.identifier.volume15en_US
dc.identifier.scopus2-s2.0-85172404937en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.biosx.2023.100392-
dc.relation.grantno116M298-
dc.authorscopusid57216562865-
dc.authorscopusid57208557682-
dc.authorscopusid57221559002-
dc.authorscopusid58145161000-
dc.authorscopusid57214225244-
dc.authorscopusid35777178800-
dc.authorscopusid8532128300-
dc.identifier.scopusqualityQ1-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.languageiso639-1en-
item.fulltextWith Fulltext-
crisitem.author.dept03.01. Department of Bioengineering-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept03.01. Department of Bioengineering-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Bioengineering / Biyomühendislik
Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
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