Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/12329
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dc.contributor.authorAlyamaç, Ayşegül-
dc.contributor.authorÖzel Taşcı, Cansu-
dc.contributor.authorGüleç, Şükrü-
dc.date.accessioned2022-08-15T18:23:24Z-
dc.date.available2022-08-15T18:23:24Z-
dc.date.issued2021-
dc.identifier.issn1096-620X-
dc.identifier.issn1557-7600-
dc.identifier.urihttps://doi.org/10.1089/jmf.2021.0034-
dc.identifier.urihttps://hdl.handle.net/11147/12329-
dc.description.abstractAnkaferd Blood Stopper (ABS) is a medicinal plant extract that has anti-inflammatory effect. Inflammatory bowel disease is a pathological condition that directly affects colon health and increases the risk of colon cancer. Especially inflammation is an important factor in the formation and progression of this disease. The aim of the study was to investigate the protective effect of ABS on colonic inflammation. Caco-2 and RAW 264.7 cells were used as a model of in vitro colonic inflammation. RAW 264.7 cells were treated with lipopolysaccharide for 12 h to induce inflammation, and an inflammatory medium (IM) was obtained. Caco-2 cells were treated with 15 mu L/mL ABS for 4 h, then incubated with IM. The cells also were incubated with 15 mu L/mL ABS and IM together for 12 h. Tumor necrosis factor alpha (TNF-alpha) protein levels were targeted in testing inflammatory condition and cyclooxygenase-2 (COX-2) mRNA level was used as a marker gene to show the possible anti-inflammatory effect of ABS in Caco-2 cells. TNF-alpha level was 26.1-fold higher than the control group. IM caused 3.2-fold increase in COX-2 expression in Caco-2 cells. Pretreatment of Caco-2 cells with ABS resulted in 3.3-fold decrease in COX-2 mRNA levels relative to IM group. Furthermore, COX-2 mRNA level reduced 4.7-fold when ABS and conditional medium were given at the same time. ABS has suppressive effect on COX-2 mRNA expression in Caco-2 cells. These results suggest that ABS might have protective and therapeutic effect for colonic inflammation.en_US
dc.description.sponsorshipIzmir Institute of Technology (IZTECH) Scientific Researchen_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert Inc.en_US
dc.relation.ispartofJournal of Medicinal Fooden_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnkaferd Blood Stopperen_US
dc.subjectCyclooxygenase-2en_US
dc.subjectInflammationen_US
dc.titleThe effect of Ankaferd Blood Stopper on colonic inflammation: An in vitro study in RAW 264.7 and Caco-2 cellsen_US
dc.typeArticleen_US
dc.departmentİzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume24en_US
dc.identifier.issue12en_US
dc.identifier.startpage1280en_US
dc.identifier.endpage1284en_US
dc.identifier.wosWOS:000726037100001en_US
dc.identifier.scopus2-s2.0-85122127117en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1089/jmf.2021.0034-
dc.identifier.pmid34847721en_US
dc.identifier.wosqualityQ3-
dc.identifier.scopusqualityQ2-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairetypeArticle-
item.fulltextNo Fulltext-
crisitem.author.dept03.08. Department of Food Engineering-
Appears in Collections:Food Engineering / Gıda Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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