Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/11440
Title: Decellularised extracellular matrix decorated PCL PolyHIPE scaffolds for enhanced cellular activity, integration and angiogenesis
Authors: Dikici, Serkan
Aldemir Dikici, Betül
MacNeil, Sheila
Claeyssens, Frederik
Keywords: Extracellular matrices
Angiogenesis
Polycaprolactone
PCL PolyHIPE scaffolds
Publisher: Royal Society of Chemistry
Abstract: Wound healing involves a complex series of events where cell-cell and cell-extracellular matrix (ECM) interactions play a key role. Wounding can be simple, such as the loss of the epithelial integrity, or deeper and more complex, reaching to subcutaneous tissues, including blood vessels, muscles and nerves. Rapid neovascularisation of the wounded area is crucial for wound healing as it has a key role in supplying oxygen and nutrients during the highly demanding proliferative phase and transmigration of inflammatory cells to the wound area. One approach to circumvent delayed neovascularisation is the exogenous use of pro-angiogenic factors, which is expensive, highly dose-dependent, and the delivery of them requires a very well-controlled system to avoid leaky, highly permeable and haemorrhagic blood vessel formation. In this study, we decorated polycaprolactone (PCL)-based polymerised high internal phase emulsion (PolyHIPE) scaffolds with fibroblast-derived ECM to assess fibroblast, endothelial cell and keratinocyte activity in vitro and angiogenesis in ex ovo chick chorioallantoic membrane (CAM) assays. Our results showed that the inclusion of ECM in the scaffolds increased the metabolic activity of three types of cells that play a key role in wound healing and stimulated angiogenesis in ex ovo CAM assays over 7 days. Herein, we demonstrated that fibroblast-ECM functionalised PCL PolyHIPE scaffolds appear to have great potential to be used as an active wound dressing to promote angiogenesis and wound healing.
URI: https://doi.org/10.1039/d1bm01262b
https://hdl.handle.net/11147/11440
ISSN: 2047-4830
2047-4849
Appears in Collections:Bioengineering / Biyomühendislik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File SizeFormat 
d1bm01262b.pdf6.71 MBAdobe PDFView/Open
Show full item record



CORE Recommender

SCOPUSTM   
Citations

22
checked on Jun 7, 2024

WEB OF SCIENCETM
Citations

21
checked on Jun 1, 2024

Page view(s)

192
checked on Jun 10, 2024

Download(s)

62
checked on Jun 10, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.