Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/11422
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dc.contributor.authorBaykara, Seden Guracar-
dc.contributor.authorSurmeli, Yusuf-
dc.contributor.authorSanli-Mohamed, Gulsah-
dc.date.accessioned2021-11-06T09:48:31Z-
dc.date.available2021-11-06T09:48:31Z-
dc.date.issued2021-
dc.identifier.issn0273-2289-
dc.identifier.issn1559-0291-
dc.identifier.urihttps://doi.org/10.1007/s12010-021-03512-0-
dc.identifier.urihttps://hdl.handle.net/11147/11422-
dc.description.abstractProteases account for approximately 60% of the enzyme market in the world, and they are used in various industrial applications including the detergent industry. In this study, production and characterization of a novel serine protease of thermophilic Geobacillus sp. GS53 from Balcova geothermal region, Izmir, Turkey, were performed. The thermostable protease was purified through ammonium sulfate precipitation and anion-exchange chromatography. The results showed that the protease had 137.8 U mg(-1) of specific activity and optimally worked at 55 C-o and pH 8. It was also active in a broad pH (4-10) and temperature (25-75 degrees C) ranges. The protease was highly stable at 85 degrees C and demonstrated relative stability at pH 4, 7, and 10. Also, the enzyme had high stability against organic solvents and surfactants; enzyme relative activity did not decrease below 81% upon preincubation for 10 min. Ca2+, Cu2+, and Zn2+ ions slightly induced protease activity. The protease was highly specific to casein, skim milk, Hammerstein casein, and BSA substrates. These results revealed that the protease might have a potential effect in a variety of industrial fields, especially the detergent industry, because of its high thermostability and stability to surfactants.en_US
dc.description.sponsorshipBiotechnology & Bioengineering Research Center at Izmir Institute of Technologyen_US
dc.description.sponsorshipThe authors would like to thank Biotechnology & Bioengineering Research Center at Izmir Institute of Technology for the facilities and technical support.en_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofApplied Biochemistry And Biotechnologyen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSerine proteaseen_US
dc.subjectGeobacillusen_US
dc.subjectThermostabilityen_US
dc.subjectSurfactanten_US
dc.titlePurification and Biochemical Characterization of a Novel Thermostable Serine Protease from Geobacillus sp. GS53en_US
dc.typeArticleen_US
dc.institutionauthor[Belirlenecek]-
dc.departmentİYTEen_US
dc.identifier.volume193en_US
dc.identifier.issue5en_US
dc.identifier.startpage1574en_US
dc.identifier.endpage1584en_US
dc.identifier.wosWOS:000612570800002en_US
dc.identifier.scopus2-s2.0-85100569779en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s12010-021-03512-0-
dc.identifier.pmid33507494en_US
dc.authorwosidSURMELI, YUSUF/AAR-3671-2020-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
crisitem.author.dept04.01. Department of Chemistry-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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