Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/11209
Title: Isolation of rosmarinic acid and methyl rosmarinate as lipoxygenase inhibitors from Salvia palaestina Benth. by activity-guided fractionation
Authors: İçen, Mehmet Sina
Gürbüz, İlhan
Bedir, Erdal
Günbatan, Tuğba
Demirci, Fatih
Keywords: Activity-guided fractionation
Anti-inflammatory activity
Lipoxygenase
Rosmarinate
Salvia palaestina
Issue Date: 2021
Publisher: Elsevier
Abstract: Salvia palaestina aqueous and methanol extracts were prepared from the aerial parts, which were evaluated for the in vitro anti-inflammatory properties using the lipoxygenase (LO) enzyme inhibition assay. While the aqueous extract showed no activity at test concentrations, a significant (p < 0.001) lipoxygenase inhibition was detected for the methanol extract with 29% inhibition. Activity guided fractionation was carried out on the methanol extract via liquid-liquid partitioning using n-hexane, dichloromethane, ethyl acetate, and n-butanol. The ethyl acetate fraction showed statistically the best inhibition among the sub-fractions with 70% inhibition (p < 0.0001). The compounds responsible for the activity were purified, and their structures were established as rosmarinic acid, and methyl rosmarinate by spectroscopic methods. IC50 values of rosmarinic acid, and methyl rosmarinate were calculated as 0.21 and 0.02 ?M, respectively. In conclusion, the in vitro anti-inflammatory potential of S. palaestina was associated to rosmarinic acid, and methyl rosmarinate, for the first time to the best of our knowledge. © 2021 SAAB
URI: http://doi.org/10.1016/j.sajb.2021.04.030
https://hdl.handle.net/11147/11209
ISSN: 0254-6299
Appears in Collections:Bioengineering / Biyomühendislik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File SizeFormat 
1-s2.0-S0254629921001642-main.pdf
  Until 2024-01-01
746.76 kBAdobe PDFView/Open    Request a copy
Show full item record

CORE Recommender

WEB OF SCIENCETM
Citations

1
checked on Dec 3, 2022

Page view(s)

488
checked on Dec 5, 2022

Download(s)

12
checked on Dec 5, 2022

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.