Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10423
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dc.contributor.authorYaman, Senatr
dc.contributor.authorTekin, Hüseyin Cumhurtr
dc.date.accessioned2021-01-24T18:43:08Z-
dc.date.available2021-01-24T18:43:08Z-
dc.date.issued2020-
dc.identifier.issn0003-2700-
dc.identifier.issn1520-6882-
dc.identifier.urihttps://doi.org/10.1021/acs.analchem.0c02479-
dc.identifier.urihttps://hdl.handle.net/10423-
dc.description.abstractMagnetic levitation, which is a magnetic phenomenon of levitating particles suspended in a paramagnetic liquid under a nonuniform magnetic field, is a powerful tool for determining densities and magnetic properties of micro- and nanoparticles. The levitation height of particles in the magnetic field depends on the magnetic susceptibility and density difference between the object and the surrounding liquid. Here, we developed a magnetic susceptibility-based protein detection scheme in a low-cost and miniaturized magnetic levitation setup consisting of two opposing magnets to create a gradient of a magnetic field, a glass capillary channel to retain the sample, and two side mirrors to monitor inside the channel. The method includes the use of polymeric microspheres as mobile assay surfaces and magnetic nanoparticles as labels. The assay was realized by capturing the target protein to the polymer microspheres. Then, magnetic nanoparticles were attached onto the resulting microsphere-protein complex, creating a significant difference in the magnetic properties of polymer microspheres compared to those without protein. The change in the magnetic properties caused a change in the levitation height of the microspheres. The levitation heights and their distribution were then correlated to the amount of target proteins. The method enabled a detection limit of similar to 110 fg/mL biotinylated bovine serum albumin in serum. With the sandwich immunoassay developed for mouse immunoglobulin G, detection limits of 1.5 ng/mL and >10 ng/mL were achieved in buffer and serum, respectively. This approach sensed the minute changes in the volume magnetic susceptibility of the microspheres with a resolution of 4.2 x 10(-8) per 1 mu m levitation height change.en_US
dc.description.sponsorshipFinancial support from The Scientific and Technological Research Council of Turkey (116M298) and Izmir Institute of Technology (2016IYTE68-BAP) are gratefully acknowledged. The authors would like to thank Engin Ozcivici, PhD, from the Department of Bioengineering, Izmir Institute of Technology, for helpful discussions and suggestions. The authors would also like to thank Katherine Willcox Ozsari from Izmir Institute of Technology for proofreading the manuscript.en_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofAnalytical Chemistryen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleMagnetic susceptibility-based protein detection using magnetic levitationen_US
dc.typeArticleen_US
dc.institutionauthorYaman, Senatr
dc.institutionauthorTekin, Hüseyin Cumhurtr
dc.departmentİzmir Institute of Technology. Bioengineeringen_US
dc.identifier.volume92en_US
dc.identifier.issue18en_US
dc.identifier.startpage12556en_US
dc.identifier.endpage12563en_US
dc.identifier.wosWOS:000572832900060en_US
dc.identifier.scopus2-s2.0-85094948266en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr
dc.identifier.doi10.1021/acs.analchem.0c02479-
dc.identifier.pmid32811142en_US
dc.relation.doi10.1021/acs.analchem.0c02479en_US
dc.coverage.doi10.1021/acs.analchem.0c02479en_US
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept03.01. Department of Bioengineering-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Bioengineering / Biyomühendislik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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