Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10376
Title: Identification and characterization of novel thermostable alpha-amylase from Geobacillus sp. GS33
Authors: Burhanoğlu, Tülin
Sürmeli, Yusuf
Şanlı Mohamed, Gülşah
Keywords: alpha-Amylase
Geobacillus
Thermostability
Issue Date: 2020
Publisher: Elsevier Ltd.
Abstract: In this study, the heterologous expression and biochemical characterization of a thermostable alpha-amylase from Geobacillus sp. GS33 was investigated. The recombinant alpha-amylase was overexpressed in Escherichia coli BL21 (lambda DE) and purified via anion exchange and size-exclusion chromatography. The purified alpha-amylase had a molecular weight of about 60 kDa, and was active in a broad range of pH 3-10 and temperature (40-90 degrees C) withmaximumactivity at pH 7-8 and 60 degrees C. The enzyme retained 50% residual activity at 65 degrees C, but only 20% at 85 degrees C after 16 h. At pH 9 and pH 7, the residual activity at 65 degrees C was 50% and 30%, respectively. The enzymewas remarkably activated by Co2+, Ca2+, Mg2+, PMSF, DTT, and Triton X-100, but partially inhibited by Cu2+, methanol, hexane, ethanol, acetone, SDS, and Tween 20. A molecular phylogeny analysis showed that the enzyme's amino acid sequence had the closest connection with an alpha-amylase from Geobacillus thermoleovorans subsp. stromboliensis nov. 3D-structure-based amino acid sequence alignments revealed that the three catalytic residues (D217, E246, D314) and the four Ca2+ ion coordination residues (N143, E177, D186, H221) were conserved in alpha-amylase from Geobacillus sp. GS33. The temperature stability and neutral pH optimum suggest that the enzyme may be useful for industrial applications. (C) 2020 Elsevier B.V. All rights reserved.
Description: PubMed: 32693140
URI: https://doi.org/10.1016/j.ijbiomac.2020.07.171
https://hdl.handle.net/10376
ISSN: 0141-8130
1879-0003
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Show full item record

CORE Recommender

SCOPUSTM   
Citations

1
checked on Aug 10, 2022

WEB OF SCIENCETM
Citations

11
checked on Jun 21, 2022

Page view(s)

600
checked on Aug 15, 2022

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.