Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10200
Title: Engineered silica nanoparticles are biologically safe vehicles to deliver drugs or genes to liver cells
Authors: Tüncel, Özge
Kahraman, Erkan
Bağcı, Gülsün
Atabey, Neşe
Özçelik, Serdar
Keywords: Cell-cycle
Colony formation
Cytotoxicity
Genotoxicity
Hemolysis
Liver
Liver cancer
Mitochondrial membrane potentials
Silica nanoparticles
Issue Date: 2021
Publisher: Elsevier Ltd.
Abstract: Engineered silica nanoparticles (SiNP) are emerging materials for medical applications. Evaluating biological responses of specific cells treated with engineered silica nanoparticles is however essential. We synthesized and characterized the physicochemical properties of silica nanoparticles with two different sizes of 10 and 100 nm (10SiNP and 100SiNP) dispersed in cell culture medium. HuH-7, an epithelial-like human hepatoblastoma cell line and SK-HEP-1, a liver sinusoidal endothelial cell line (LSEC) are employed to evaluate their biological responses for the SiNP treatment. Primary human lymphocytes are used to assess genotoxicity recommended by OECD guidelines while erythrocytes are used to assess hemolytic activity. The engineered silica nanoparticles are not able to produce radical species, to alter the mitochondrial membrane potential, and induce any adverse effects on cell proliferation. The colony formation ability of HuH-7 hepatoblastoma cells was not affected following the SiNP treatment. Furthermore, SiNPs do not induce hemolysis of red blood cells and are not genotoxic. These findings suggest that SiNPs regardless of the size, amount, and incubation time are biologically safe vehicles to deliver drugs or genes to the liver. © 2020 Elsevier B.V.
URI: https://doi.org/10.1016/j.msec.2020.111585
https://hdl.handle.net/10200
ISSN: 0928-4931
1873-0191
Appears in Collections:Chemistry / Kimya
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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