Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/7630
Full metadata record
DC FieldValueLanguage
dc.contributor.authorİlgü, Hüseyin-
dc.contributor.authorSürmeli, Yusuf-
dc.contributor.authorŞanlı Mohamed, Gülşah-
dc.date.accessioned2020-01-27T12:19:35Z
dc.date.available2020-01-27T12:19:35Z
dc.date.issued2018-09en_US
dc.identifier.citationİlgü, H., Sürmeli, Y., and Şanlı Mohamed, G. (2018). A thermophilic α-l-Arabinofuranosidase from Geobacillus vulcani GS90: heterologous expression, biochemical characterization, and its synergistic action in fruit juice enrichment. European Food Research and Technology, 244(9), 1627-1636. doi:10.1007/s00217-018-3075-7en_US
dc.identifier.issn1438-2377
dc.identifier.issn1438-2377-
dc.identifier.urihttps://doi.org/10.1007/s00217-018-3075-7
dc.identifier.urihttps://hdl.handle.net/11147/7630
dc.description.abstractα-l-Arabinofuranosidases with an orchestral action of xylanolytic enzymes degrades the xylan in plant cell wall. In this study, heterologous expression, biochemical characterization, and synergistic action of α-l-Arabinofuranosidase from previously identified.Geobacillus vulcani GS90 (GvAbf) was investigated. The recombinant α-l-Arabinofuranosidase was overexpressed in Escherichia coli BL21 (λDE) and purified via His-tag Ni-affinity and size-exclusion chromatography. Optimum activity of the purified α-l-Arabinofuranosidase was obtained at pH 5 and at 70 °C. The GvAbf was active in a broad pH and temperature ranges; pH 4–9 and 30–90 °C, respectively. In addition, it retained most of its activity after an hour incubation at 70 °C and remained relatively stable at pH 3–6. GvAbf was quite stable against various metal ions. The kinetic parameters of GvAbf was obtained as Vmax and Km; 200 U/mg and 0.2 mM with p-nitrophenyl-α-l-arabinofuranoside and 526 U/mg and 0.1 mM with sugar beet arabinan, respectively. The synergistic action of GvAbf was studied with commercially available xylanase on juice enrichment of apples, grapes, oranges, and peaches. The best juice enrichment in terms of clarity, reducing sugar content, and yield, was achieved with GvAbf and xylanase together compared to treatment with xylanase and GvAbf alone in all fruits. The treatment with GvAbf and xylanase together lead to an increased juice yield by 26.56% (apple), 30.88% (grape), 40.00% (orange) and 32.20% (peach) as well as having a significant effect on juice clarity by an increase of % transmittance 47.26, 25.98, 41.77, and 44.97, respectively. The highest reducing sugar level of fruit juices also obtained with GvAbf and xylanase together compared to treatment with xylanase and GvAbf alone in all types of fruits. GvAbf and xylanase together as simultaneous synergistic manner may have an exciting potential for application in fruit juice processing.en_US
dc.language.isoengen_US
dc.publisherSpringer Verlagen_US
dc.relation.ispartofEuropean Food Research and Technologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFruit juicesen_US
dc.subjectGeobacillus vulcanien_US
dc.subjectSynergistic actionen_US
dc.subjectα-l-Arabinofuranosidaseen_US
dc.subjectEscherichia colien_US
dc.titleA thermophilic ?-l-Arabinofuranosidase from Geobacillus vulcani GS90: heterologous expression, biochemical characterization, and its synergistic action in fruit juice enrichmenten_US
dc.typeArticleen_US
dc.authorid0000-0003-0282-4428en_US
dc.departmentIzmir Institute of Technology. Chemistryen_US
dc.identifier.volume244en_US
dc.identifier.issue9en_US
dc.identifier.startpage1627en_US
dc.identifier.endpage1636en_US
dc.identifier.wosWOS:000440731100011
dc.identifier.scopusSCOPUS:2-s2.0-85045910005
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s00217-018-3075-7-
dc.relation.doi10.1007/s00217-018-3075-7en_US
dc.coverage.doi10.1007/s00217-018-3075-7en_US
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.deptDepartment of Chemistry-
Appears in Collections:Chemistry / Kimya
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Files in This Item:
File Description SizeFormat 
İlgü2018_Article.pdfMakale (Article)971.89 kBAdobe PDFThumbnail
View/Open
Show simple item record

CORE Recommender

SCOPUSTM   
Citations

5
checked on Oct 23, 2021

WEB OF SCIENCETM
Citations

8
checked on Oct 23, 2021

Page view(s)

32
checked on Oct 27, 2021

Download(s)

62
checked on Oct 27, 2021

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.