Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6757
Title: Differentially expressed tRNA-derived small RNAs co-sediment primarily with non-polysomal fractions in Drosophila
Authors: Göktaş, Çağdaş
Yiğit, Hatice
Coşacak, Mehmet İlyas
Akgül, Bünyamin
Keywords: Drosophila
Polysome
TRNA
TRF
Issue Date: Nov-2017
Publisher: MDPI Multidisciplinary Digital Publishing Institute
Source: Göktaş, Ç., Yiğit, H., Coşacak, M. İ., and Akgül, B. (2017). Differentially expressed tRNA-derived small RNAs co-sediment primarily with non-polysomal fractions in Drosophila. Genes, 8(11). doi:10.3390/genes8110333
Abstract: Recent studies point to the existence of poorly characterized small regulatory RNAs generated from mRNAs, rRNAs and tRNAs. To explore the subcellular location of tRNA-derived small RNAs, 0–1 and 7–8 h Drosophila embryos were fractionated on sucrose density gradients. Analysis of 12,553,921 deep-sequencing reads from unfractionated and fractionated Drosophila embryos has revealed that tRFs, which are detected mainly from the 5’ends of tRNAs, co-sediment with the non-polysomal fractions. Interestingly, the expression levels of a subset of tRFs change temporally following thematernal-to-zygotic transition in embryos. We detected non-polysomal association of tRFs in S2 cells as well. Differential tRF expression pattern points to developmental significance at the organismal level. These results suggest that tRFs are associated primarily with the non-polysomal complexes in Drosophila embryos and S2 cells.
URI: http://doi.org/10.3390/genes8110333
http://hdl.handle.net/11147/6757
ISSN: 2073-4425
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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