Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6491
Title: Stimuli responsive polymer-based strategies for polynucleotide delivery
Authors: Uz, Metin
Alsoy Altınkaya, Sacide
Mallapragada, Surya K.
Alsoy Altınkaya, Sacide
Izmir Institute of Technology. Chemical Engineering
Keywords: Functional polymers
Stimuli responsive polymers
Gene delivery
CRISPR/Cas9
Small interfering RNA
Issue Date: Aug-2017
Publisher: Cambridge University Press
Source: Uz, M., Alsoy Altınkaya, S., and Mallapragada, S. K. (2017). Stimuli responsive polymer-based strategies for polynucleotide delivery. Journal of Materials Research, 32(15), 2930-3953. doi:10.1557/jmr.2017.116
Abstract: In recent years, stimuli responsive polymer based gene delivery vehicle design for cancer treatment and treatment of other genetic disorders has received extensive attention. Early studies focusing on DNA delivery have been facilitated by functional polymers and this area has seen further growth spurred by recent gene silencing strategies developed for small RNA [i.e., small interfering RNA (siRNA) or micro RNA (miRNA)] delivery. DNA and small RNAs possess analogous properties; however, their explicit differences define the specific challenges associated with the delivery route and the design of functional materials to overcome distinct challenges. Apart from classical gene delivery, the recent advances in genome editing have revealed the necessity of new delivery devices for genome editing tools. A system involving CRISPR (clustered, regularly interspaced, short palindromic repeats) and an endonuclease CRISPR-associated protein 9 (Cas9) coupled with a short, single-guide RNA (sgRNA) has emerged as a promising tool for genome editing along with functional delivery systems. For all these nucleic acid based treatments, the internal or external physiochemical changes in the biological tissue/cells play a major role in the design of stimuli responsive delivery materials for both in vitro and in vivo applications. This review emphasizes the recent advances in the use of pH, temperature, and redox potential-responsive polymers overcoming hurdles for delivery of gene and gene editing tools for both in vitro and in vivo applications. Specifically the chapter focuses on recently proposed delivery strategies, types of delivery systems, and polymer synthesis/modification methods. The recent advances in CRISPR/Cas9-sgRNA technology and delivery are also described in a separate section. The review ends with current clinical trials, concluding remarks, and future perspectives.
URI: http://doi.org/10.1557/jmr.2017.116
http://hdl.handle.net/11147/6491
ISSN: 0884-2914
2044-5326
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
6491.pdfİnceleme (Review)710.1 kBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

SCOPUSTM   
Citations

9
checked on Jul 24, 2021

WEB OF SCIENCETM
Citations

5
checked on Jul 24, 2021

Page view(s)

16
checked on Jul 29, 2021

Download(s)

10
checked on Jul 29, 2021

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.