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Title: Development of genomic simple sequence repeat markers in faba bean by next-generation sequencing
Authors: Abuzayed, Mazen A.
Göktay, Mehmet
Allmer, Jens
Doğanlar, Sami
Frary, Anne
Keywords: Genetic diversity
Genomic SSRs
Illumina sequencing
Population structure
Vicia faba L.
Publisher: Springer Verlag
Source: Abuzayed, M. A., Göktay, M., Allmer, J., Doğanlar, S., and Frary, A. (2017). Development of genomic simple sequence repeat markers in faba bean by next-generation sequencing. Plant Molecular Biology Reporter, 35(1), 61-71. doi:10.1007/s11105-016-1003-1
Abstract: Faba bean (Vicia faba L.) is an important food legume crop with a huge genome. Development of genetic markers for faba bean is important to study diversity and for molecular breeding. In this study, we used Next Generation Sequencing (NGS) technology for the development of genomic simple sequence repeat (SSR) markers. A total of 14,027,500 sequence reads were obtained comprising 4,208 Mb. From these reads, 56,063 contigs were assembled (16,367 Mb) and 2138 SSRs were identified. Mono and dinucleotides were the most abundant, accounting for 57.5 % and 20.9 % of all SSR repeats, respectively. A total of 430 primer pairs were designed from contigs larger than 350 nucleotides and 50 primers pairs were tested for validation of SSR locus amplification. Nearly all (96 %) of the markers were found to produce clear amplicons and to be reproducible. Thirty-nine SSR markers were then applied to 46 faba bean accessions from worldwide origins, resulting in 161 alleles with 87.5 % polymorphism, and an average of 4.1 alleles per marker. Gene diversity (GD) of the markers ranged from 0 to 0.48 with an average of 0.27. Testing of the markers showed that they were useful in determining genetic relationships and population structure in faba bean accessions.
ISSN: 0735-9640
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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