Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5936
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dc.contributor.authorTelli, Nihat-
dc.contributor.authorGüner, Ahmet-
dc.contributor.authorSoyer Dönmez, Ferda-
dc.contributor.authorÖzdemir, Özgün Öykü-
dc.date.accessioned2017-07-17T07:23:55Z-
dc.date.available2017-07-17T07:23:55Z-
dc.date.issued2016-
dc.identifier.citationTelli, N., Güner, A., Soyer Dönmez, F., and Özdemir, Ö. Ö. (2016). Detection of the contamination sources of Listeria monocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. Turkish Journal of Veterinary and Animal Sciences, 40(5), 630-636. doi:10.3906/vet-1511-59en_US
dc.identifier.issn1300-0128-
dc.identifier.issn1303-6181-
dc.identifier.urihttp://doi.org/10.3906/vet-1511-59-
dc.identifier.urihttp://hdl.handle.net/11147/5936-
dc.description.abstractThis study was conducted to determine the contamination sources, serotyping profiles, and antibiotic resistance patterns of Listeria monocytogenes isolated during the production of pickled white cheese. The genetic-relatedness of the isolates to EGD SLCC (5835) (1/2a, lineage II) and ATCC (13932) (4b, lineage I) reference strains was also determined with pulsed-field gel electrophoresis (PFGE) as a result of digestions with AscI and ApaI enzymes. Samples were collected from 16 different points in the production process of 4 different plants at 3 different times. Among the 192 samples examined, 17 (8.85%) were determined to be contaminated with Listeria spp. Three isolates (3.53%) obtained from raw milk, wall/ground, and press cases were identified as L. monocytogenes via the conventional culture method and confirmed by polymerase chain reaction. These isolates were found to belong to serotype 4b. According to antibiotic resistance testing against 10 antibiotics (ampicillin, gentamicin, erythromycin, tetracycline, chloramphenicol, cefalotin, streptomycin, vancomycin, penicillin, and sulfamethoxazole/trimethoprim), it was determined that isolates from raw milk and press cases were resistant to erythromycin. PPGE band patterns of the isolates displayed indistinguishable with AscI and 80%-94% homology with ApaI. The isolates were observed to display high homology to ATCC (13932) and lower homology to EGD SLCC (5835) obtained by both enzymes.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Coordination Unit (09102044)en_US
dc.language.isoenen_US
dc.publisherTUBITAKen_US
dc.relation.ispartofTurkish Journal of Veterinary and Animal Sciencesen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntibiotic resistanceen_US
dc.subjectListeria monocytogenesen_US
dc.subjectPickled white cheeseen_US
dc.subjectSerotypingen_US
dc.titleDetection of the contamination sources of Listeria monocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis methoden_US
dc.typeArticleen_US
dc.authoridTR114400en_US
dc.authoridTR115395en_US
dc.institutionauthorSoyer Dönmez, Ferda-
dc.institutionauthorÖzdemir, Özgün Öykü-
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume40en_US
dc.identifier.issue5en_US
dc.identifier.startpage630en_US
dc.identifier.endpage636en_US
dc.identifier.wosWOS:000387808500016
dc.identifier.scopusSCOPUS:2-s2.0-84995917626
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.3906/vet-1511-59-
dc.relation.doi10.3906/vet-1511-59en_US
dc.coverage.doi10.3906/vet-1511-59en_US
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextWith Fulltext-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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