Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5681
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dc.contributor.authorUz, Metin-
dc.contributor.authorMallapragada, Surya K.-
dc.contributor.authorAlsoy Altınkaya, Sacide-
dc.date.accessioned2017-06-02T08:50:25Z-
dc.date.available2017-06-02T08:50:25Z-
dc.date.issued2015-
dc.identifier.citationUz, M., Mallapragada, S. K., and Alsoy Altınkaya, S. (2015). Responsive pentablock copolymers for siRNA delivery. RSC Advances, 5(54), 43515-43527. doi:10.1039/c5ra06252gen_US
dc.identifier.issn2046-2069-
dc.identifier.urihttp://doi.org/10.1039/c5ra06252g-
dc.identifier.urihttp://hdl.handle.net/11147/5681-
dc.description.abstractIn this study, temperature and pH responsive cationic and amphiphilic pentablock copolymers, which consist of the temperature responsive triblock Pluronic F127 sandwiched between pH responsive PDEAEM (poly(2-diethylaminoethyl methacrylate)) end blocks, were used for the first time in the development of polyplex and gold nanoparticle (AuNP) based multicomponent siRNA delivery systems (MCSs). Copolymers in both systems protected siRNA from external effects, provided cell entry and endosomal escape. The thermoreversible micellization of the hydrophobic PPO block facilitated the cellular entry while the PDEAEM blocks enhanced the endosomal escape through protonated tertiary amine groups by pH buffering. The synergistic advantages of the different blocks showed an enhanced effect in the MCSs due to attachment and surface configuration reasons. The siRNA transfection efficiency of MCSs against luciferase expressing SKOV3 cells was 15% higher than both the polyplexes alone and the commercial siRNA transfection agent Lipofectamine RNAiMax at the same applied dose, without any toxicity. The results indicated that the multicomponent systems based on the responsive cationic pentablock copolymers and gold nanoparticles have promising potential as an efficient siRNA delivery vector for future applications.en_US
dc.description.sponsorshiphe Scientific and Technological Research Council of Turkey (TUBITAK) Graduate Scholarship Programme; Izmir Institute of Technology (2009IYTE01); US Army Medical Research and Materiel Command (W81XWH-10-1-0806); Stanley Endowed Chair in Interdisciplinary Engineeringen_US
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofRSC Advancesen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGolden_US
dc.subjectHydrogelsen_US
dc.subjectMetal nanoparticlesen_US
dc.subjectMobile securityen_US
dc.subjectsiRNA deliveryen_US
dc.subjectCopolymersen_US
dc.subjectLiposomesen_US
dc.titleResponsive pentablock copolymers for siRNA deliveryen_US
dc.typeArticleen_US
dc.authoridTR2091en_US
dc.institutionauthorUz, Metin-
dc.institutionauthorAlsoy Altınkaya, Sacide-
dc.departmentİzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume5en_US
dc.identifier.issue54en_US
dc.identifier.startpage43515en_US
dc.identifier.endpage43527en_US
dc.identifier.wosWOS:000354964600056en_US
dc.identifier.scopus2-s2.0-84929591240en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1039/c5ra06252g-
dc.relation.doi10.1039/c5ra06252gen_US
dc.coverage.doi10.1039/c5ra06252gen_US
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.fulltextWith Fulltext-
crisitem.author.dept03.02. Department of Chemical Engineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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