Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5629
Title: Preparation and characterization of polyacrylonitrile membranes modified with polyelectrolyte deposition for separating similar sized proteins
Authors: Yaşar Mahlıçlı, Filiz
Alsoy Altınkaya, Sacide
Yürekli, Yılmaz
Yaşar Mahlıçlı, Filiz
Alsoy Altınkaya, Sacide
Izmir Institute of Technology. Chemical Engineering
Keywords: Lysozyme
Myoglobin
Polyacrylonitrile
Polyelectrolytes
Ultrafiltration
Issue Date: Oct-2012
Publisher: Elsevier Ltd.
Source: Yaşar Mahlıçlı, F., Alsoy Altınkaya, S. and Yürekli, Y. (2012). Preparation and characterization of polyacrylonitrile membranes modified with polyelectrolyte deposition for separating similar sized proteins. Journal of Membrane Science, 415-416, 383-390. doi:10.1016/j.memsci.2012.05.028
Abstract: One of the challenges faced by ultrafiltration membranes is to separate proteins with a small difference in their molecular weights. Recently, some researchers tried to overcome this problem by using charged membranes. This study examined the use of layer by layer deposition of polyelectrolytes on the chemically-modified polyacyronitrile membrane to increase the selectivity of the ultrafiltration. The membranes were prepared by wet-phase inversion technique and polyethylenimine (PEI) and alginate (ALG) were chosen as cationic and anionic polyelectrolytes for the modification of the surfaces. Sieving coefficient data were obtained with myoglobin and lysozyme as model proteins. The influences of solution pH, ionic strengths of the protein and polyelectrolyte solution and the number of polyelectrolyte bilayers on both selectivity and throughput were investigated. The highest selectivity and throughput were achieved with the 1-bilayer PEI-ALG coated polyacrylonitrile (PAN) membrane. Increasing the number of coating bilayers or the ionic strength of the protein solution or adding salt into the polyelectrolyte coating solution decreased both the maximum selectivity and throughput of the modified membranes.
URI: http://dx.doi.org/10.1016/j.memsci.2012.05.028
http://hdl.handle.net/11147/5629
ISSN: 0376-7388
1873-3123
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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