Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5621
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dc.contributor.authorSevimli, Sema-
dc.contributor.authorSagnella, Sharon-
dc.contributor.authorKavallaris, Maria-
dc.contributor.authorBulmuş, Volga-
dc.contributor.authorDavis, Thomas P.-
dc.date.accessioned2017-05-29T07:09:33Z-
dc.date.available2017-05-29T07:09:33Z-
dc.date.issued2012-08-
dc.identifier.citationSevimli, S., Sagnella, S., Kavallaris, M., Bulmuş, V. and Davis, T. P. (2012). Synthesis, self-assembly and stimuli responsive properties of cholesterol conjugated polymers. Polymer Chemistry, 3(8), 2057-2069. doi:10.1039/c2py20112gen_US
dc.identifier.issn1759-9954-
dc.identifier.issn1759-9962-
dc.identifier.urihttp://dx.doi.org/10.1039/c2py20112g-
dc.identifier.urihttp://hdl.handle.net/11147/5621-
dc.description.abstractReversible addition-fragmentation chain transfer (RAFT) polymerization was used to generate well-defined pH-responsive biofunctional polymers as potential 'smart' gene delivery systems. A series of five poly(dimethylamino ethyl methacrylate-co-cholesteryl methacrylate) P(DMAEMA-co-CMA) statistical copolymers, with similar molecular weights and varying cholesterol content, were prepared. The syntheses, compositions and molecular weight distributions for P(DMAEMA-co-CMA) were monitored by nuclear magnetic resonance (NMR), solid-state NMR and gel permeation chromatography (GPC) evidencing well-defined polymeric structures with narrow polydispersities. Aqueous solution properties of the copolymers were investigated using turbidimetry and light scattering to determine hydrodynamic diameters and zeta potentials associated with the phase transition behaviour of P(DMAEMA-co-CMA) copolymers. UV-Visible spectroscopy was used to investigate the pH-responsive behaviour of copolymers. Hydrodynamic radii were measured in the range 10-30 nm (pH, temperature dependent) by dynamic light scattering (DLS). Charge studies indicated that P(DMAEMA-co-CMA) polymers have an overall cationic charge, mediated by pH. Potentiometric studies revealed that the buffering capacity and pK a values of polymers were dependent on cholesterol content as well as on cationic charge. The buffering capacity increased with increasing charge ratio, overall demonstrating transitions in the pH endosomal region for all five copolymeric structures. Cell viability assay showed that the copolymers displayed increasing cytotoxicity with decreasing number of cholesterol moieties. These preliminary results show the potential of these well-defined P(DMAEMA-co-CMA) polymers as in vitro siRNA delivery agents.en_US
dc.description.sponsorshipDVCR UNSW; UNSWen_US
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofPolymer Chemistryen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPolydispersityen_US
dc.subjectPolymeric structuresen_US
dc.subjectPH-responsiveen_US
dc.subjectCholesterol contenten_US
dc.subjectStatistical copolymersen_US
dc.titleSynthesis, self-assembly and stimuli responsive properties of cholesterol conjugated polymersen_US
dc.typeArticleen_US
dc.authoridTR181383en_US
dc.institutionauthorBulmuş, Volga-
dc.departmentIzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume3en_US
dc.identifier.issue8en_US
dc.identifier.startpage2057en_US
dc.identifier.endpage2069en_US
dc.identifier.wosWOS:000305964400019en_US
dc.identifier.scopus2-s2.0-84867463137en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1039/c2py20112g-
dc.relation.doi10.1039/c2py20112gen_US
dc.coverage.doi10.1039/c2py20112gen_US
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.languageiso639-1en-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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