Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5057
Title: Synthesis of heterotelechelic polymers with affinity to glutathione-S-transferase and biotin-tagged proteins by RAFT polymerization and thiol-ene reactions
Authors: Huang, Xin
Boyer, Cyrille
Davis, Thomas P.
Bulmuş, Volga
Keywords: Bioaffinity
Bovine serum albumins
Cancer markers
Chain transfer agents
Functional polymers
Issue Date: Jul-2011
Publisher: Royal Society of Chemistry
Source: Huang, X., Boyer, C., Davis, T. P., and Bulmus, V. (2011). Synthesis of heterotelechelic polymers with affinity to glutathione-S-transferase and biotin-tagged proteins by RAFT polymerization and thiol-ene reactions. Polymer Chemistry, 2(7), 1505-1512. doi:10.1039/c1py00049g
Abstract: α-Glutathione (GSH), ω-biotin functionalized poly(N-isopropylacrylamide) (PNIPAAm) was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization using a new R-group allyl functionalized trithiocarbonate chain transfer agent (CTA) and thiol-ene reactions. GPC and 1H NMR results indicated that the allyl group had no adverse effect on the RAFT-controlled polymerization of NIPAAm and PEG-A, and the new CTA could efficiently control the polymerizations. Employing radical thiol-ene and Michael addition reactions, heterotelechelic α-allyl, ω-carboxylic acid-PNIPAAm was first aminolyzed in the presence of maleimide-modified biotin and subsequently reacted with GSH via radical thiol-ene addition to yield α-GSH, ω-biotin functionalized PNIPAAm. Glutathione S-transferase (GST) and streptavidin (SAv) were coupled in solution with heterofunctional PNIPAAm via bioaffinity interactions. Separately, α-GSH, ω-biotin functionalized PNIPAAm was further shown to bind GST-tagged Rac1, a potential cancer marker, and biotin-tagged bovine serum albumin (BSA).
URI: https://doi.org/10.1039/c1py00049g
http://hdl.handle.net/11147/5057
ISSN: 1759-9954
1759-9962
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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