Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5015
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBüyükkileci, Ali Oğuz-
dc.contributor.authorTarı, Canan-
dc.contributor.authorFernandez-Lahore, Marcello-
dc.date.accessioned2017-03-09T07:08:48Z-
dc.date.available2017-03-09T07:08:48Z-
dc.date.issued2011-08-
dc.identifier.citationBüyükkileci, A. O., Tarı, C., and Fernandez-Lahore, M. (2011). Enhanced production of exo-polygalacturonase from agro-based products by Aspergillus sojae. BioResources, 6(3), 3452-3468.en_US
dc.identifier.issn1930-2126-
dc.identifier.urihttp://hdl.handle.net/11147/5015-
dc.description.abstractAspergillus sojae has been previously shown to produce exo-polygalacturonase (exo-PG) in synthetic media, where the potential of the organism to utilize agricultural substrates was not considered so far. In this study, the utilization of agro-based products was taken into account in the enhanced production of exo-PG using an A. sojae mutant by applying statistical design methods. Complex sources (orange peel, wheat bran, and corn meal), simple sugar sources (glucose, maltrin, and sugar beet syrup), and two phosphate salts were screened using D-optimal design method. Orange peel yielded the highest exo-PG activity with all simple sugars and phosphate sources. According to the results of response surface methodology (RSM), the optimum concentrations of orange peel, sugar beet syrup, and (NH 4) 2SO 4 were found to be 10, 60, and 8 g L -1, respectively. The exo-PG activity under these conditions was 145.4 U m L -1 in shake flask cultures. In bioreactor studies enzyme production was induced at low pH values; thus highest production was obtained under uncontrolled pH conditions, in which the pH dropped to 2.0 in 72 h. As a result high exo-PG could be produced by an A. sojae mutant using a cost-effective medium containing agro-industrial substrates. Another important advantageous outcome was the low optimal pH, which is especially desired in industrial fermentations prone to contamination problems. In fact this highlights the easy adaptation of this fermentation to industrial scales.en_US
dc.description.sponsorshipTUBITAK (Turkey) and BMBF (Germany) through the Intensified Cooperation Project of IntenC 107O602en_US
dc.language.isoenen_US
dc.publisherNorth Carolina Universityen_US
dc.relation.ispartofBioResourcesen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAspergillus sojaeen_US
dc.subjectMedium optimizationen_US
dc.subjectAgro-industrial substratesen_US
dc.subjectExo-polygalacturonaseen_US
dc.subjectFermentationen_US
dc.titleEnhanced production of exo-polygalacturonase from agro-based products by Aspergillus sojaeen_US
dc.typeArticleen_US
dc.authoridTR29392en_US
dc.institutionauthorBüyükkileci, Ali Oğuz-
dc.institutionauthorTarı, Canan-
dc.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume6en_US
dc.identifier.issue3en_US
dc.identifier.startpage3452en_US
dc.identifier.endpage3468en_US
dc.identifier.wosWOS:000295864500091
dc.identifier.scopusSCOPUS:2-s2.0-80051571902
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextWith Fulltext-
crisitem.author.deptDepartment of Food Engineering-
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Files in This Item:
File Description SizeFormat 
5015.pdfMakale360.88 kBAdobe PDFThumbnail
View/Open
Show simple item record

CORE Recommender

Page view(s)

20
checked on Dec 8, 2021

Download(s)

10
checked on Dec 8, 2021

Google ScholarTM

Check


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.