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dc.contributor.authorAydın, Ali
dc.contributor.authorSudağıdan, Mert
dc.contributor.authorMuratoğlu, Karlo
dc.date.accessioned2017-03-06T12:19:53Z
dc.date.available2017-03-06T12:19:53Z
dc.date.issued2011-08
dc.identifier.citationAydın, A., Sudağıdan, M., and Muratoğlu, K. (2011). Prevalence of staphylococcal enterotoxins, toxin genes and genetic-relatedness of foodborne Staphylococcus aureus strains isolated in the Marmara Region of Turkey. International Journal of Food Microbiology, 148(2), 99-106. doi:10.1016/j.ijfoodmicro.2011.05.007en_US
dc.identifier.issn0168-1605
dc.identifier.urihttp://doi.org/10.1016/j.ijfoodmicro.2011.05.007
dc.identifier.urihttp://hdl.handle.net/11147/4982
dc.description.abstractStaphylococcus aureus is a major foodborne pathogen and it has the ability to produce a number of extracellular toxins. We analyzed 1070 food samples obtained from retail markets and dairy farms in the Marmara Region of Turkey for the presence of S. aureus. Out of 147 isolates, 92 (62.6%) were enterotoxigenic. PCR was used to investigate the presence of staphylococcal enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq and seu), exfoliative toxin genes (eta and etb) and the toxic - shock syndrome toxin gene (tst). The PCR results showed that 53.3% of the isolates contained staphylococcal enterotoxin-like (SEl) toxin genes (seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq and seu) which were more frequent than classical enterotoxin genes (sea to see). Furthermore, seo, sei, sem, seg, seu and sec were found in 37.0, 32.7, 30.4, 29.3, 29.3 and 27.2% of the isolates, respectively. The tst gene was detected and confirmed by DNA sequencing in 9 isolates. The presence of eta and etb were not found in the isolates. Enterotoxigenic capabilities of isolates with SEA-SEE were investigated by ELISA. Enterotoxigenic S. aureus isolates produced one to three enterotoxins, with the most frequently produced types being enterotoxin A and C. There was a correlation of 72.1% between production of a specific toxin and the presence of the respective genes. PFGE analysis was used to identify genetic-relatedness of enterotoxigenic S. aureus isolates and the results revealed that 13 groups of isolates from different or the same origin that contained the same genes showed 100% homology with indistinguishable band patterns. The other enterotoxigenic isolates showed related band patterns with 72-86% homology in sea-, 61-90% homology in sec-, 80-96% homology in seh-, and 69-96% homology in sep-positive isolates. To our knowledge, this is the first study to examine enterotoxins and related gene contents of S. aureus food isolates in the Marmara Region of Turkey.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (107T266); Istanbul University (UDP-3645/13042009)en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.ijfoodmicro.2011.05.007en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectELISAen_US
dc.subjectEnterotoxin genesen_US
dc.subjectEnterotoxinsen_US
dc.subjectPFGEen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectPCR
dc.titlePrevalence of staphylococcal enterotoxins, toxin genes and genetic-relatedness of foodborne Staphylococcus aureus strains isolated in the Marmara Region of Turkeyen_US
dc.typearticleen_US
dc.contributor.institutionauthorSudağıdan, Mert
dc.relation.journalInternational Journal of Food Microbiologyen_US
dc.contributor.departmentIzmir Institute of Technology. Biotechnology and Bioengineering Research and Application Center (IZTECH BIYOMER)en_US
dc.identifier.volume148en_US
dc.identifier.issue2en_US
dc.identifier.startpage99en_US
dc.identifier.endpage106en_US
dc.identifier.wosWOS:000293108400005
dc.identifier.scopusSCOPUS:2-s2.0-79959508145
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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