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dc.contributor.authorİlgü, Hüseyin
dc.contributor.authorTuran, Taylan
dc.contributor.authorŞanlı Mohamed, Gülşah
dc.date.accessioned2017-03-02T07:49:07Z
dc.date.available2017-03-02T07:49:07Z
dc.date.issued2011-09
dc.identifier.citationİlgü, H., Turan, T., and Şanlı Mohamed, G. (2011). Preparation, characterization and optimization of chitosan nanoparticles as carrier for immobilization of thermophilic recombinant esterase. Journal of Macromolecular Science, Part A: Pure and Applied Chemistry, 48(9), 713-721. doi:10.1080/10601325.2011.596050en_US
dc.identifier.issn1060-1325
dc.identifier.urihttp://doi.org/10.1080/10601325.2011.596050
dc.identifier.urihttp://hdl.handle.net/11147/4947
dc.description.abstractImmobilization of biologically important molecules on myriad nano-sized materials has attracted great attention. Through this study, thermophilic esterase enzyme was obtained using recombinant DNA technology and purified applying one-step His-Select HF nickel affinity gel. The synthesis of chitosan was achieved from chitin by deacetylation process and degree of deacetylation was calculated as 89% by elemental analysis. Chitosan nanoparticles were prepared based on the ionic gelation of chitosan with tripolyphosphate anions. The physicochemical properties of the chitosan and chitosan nanoparticles were determined by several methods including SEM (Scanning Electron Microscopy), FT-IR (Fourier Transform Infrared Spectroscopy) and DLS (Dynamic Light Scattering). The morphology of chitosan nanoparticles was spherical and the nanospheres' average diameter was 75.3 nm. The purified recombinant esterase was immobilized efficiently by physical adsorption onto chitosan nanoparticles and effects of various immobilization conditions were investigated in details to develope highly cost-effective esterase as a biocatalyst to be utilized in biotechnological purposes. The optimal conditions of immobilization were determined as follows; 1.0 mg/mL of recombinant esterase was immobilized on 1.5 mg chitosan nanoparticles for 30 min at 60C, pH 7.0 under 100 rpm stirring speed. Under optimized conditions, immobilized recombinant esterase activity yield was 88.5%. The physicochemical characterization of enzyme immobilized chitosan nanoparticles was analyzed by SEM, FT-IR and AFM (Atomic Force Microscopy).en_US
dc.language.isoengen_US
dc.publisherTaylor & Francisen_US
dc.relation.isversionof10.1080/10601325.2011.596050en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEsteraseen_US
dc.subjectChitosanen_US
dc.subjectImmobilizationen_US
dc.subjectNanoparticlesen_US
dc.titlePreparation, characterization and optimization of chitosan nanoparticles as carrier for immobilization of thermophilic recombinant esteraseen_US
dc.typearticleen_US
dc.contributor.authorIDTR115002en_US
dc.contributor.iztechauthorİlgü, Hüseyin
dc.contributor.iztechauthorTuran, Taylan
dc.contributor.iztechauthorŞanlı Mohamed, Gülşah
dc.relation.journalJournal of Macromolecular Science, Part A: Pure and Applied Chemistryen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Kimya Bölümüen_US
dc.identifier.volume48en_US
dc.identifier.issue9en_US
dc.identifier.startpage713en_US
dc.identifier.endpage721en_US
dc.identifier.wosWOS:000299745500006
dc.identifier.scopusSCOPUS:2-s2.0-79961043069
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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