Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4122
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dc.contributor.authorOrr, Galya-
dc.contributor.authorHu, Dehong-
dc.contributor.authorÖzçelik, Serdar-
dc.contributor.authorWiley, H. Steven-
dc.contributor.authorColson, Steven D.-
dc.contributor.authorOpresko, Lee K.-
dc.date.accessioned2014-07-25T03:29:53Z
dc.date.available2014-07-25T03:29:53Z
dc.date.issued2013-10-01en_US
dc.identifier.citationOrr, G., Hu, D., Özçelik, S., Opresko, L., Wiley, H., & Colson, S. (2005). Cholesterol Dictates the Freedom of EGF Receptors and HER2 in the Plane of the Membrane. Biophysical Journal, 89(2), 1362–1373. doi:10.1529/biophysj.104.056192en_US
dc.identifier.issn1362-1373-
dc.identifier.urihttp://dx.doi.org/10.1529/biophysj.104.056192en_US
dc.identifier.urihttp://hdl.handle.net/11147/4122
dc.description.abstractThe flow of information through the epidermal growth factor receptor (EGFR) is shaped by molecular interactions in the plasma membrane. The EGFR is associated with lipid rafts, but their role in modulating receptor mobility and subsequent interactions is unclear. To investigate the role of nanoscale rafts in EGFR dynamics, we used single-molecule fluorescence imaging to track individual receptors and their dimerization partner, human epidermal growth factor receptor 2 (HER2), in the membrane of human mammary epithelial cells. We found that the motion of both receptors was interrupted by dwellings within nanodomains. EGFR was significantly less mobile than HER2. This difference was likely due to F-actin because its depolymerization led to similar diffusion patterns between the EGFR and HER2. Manipulations of membrane cholesterol content dramatically altered the diffusion pattern of both receptors. Cholesterol depletion led to almost complete confinement of the receptors, whereas cholesterol enrichment extended the boundaries of the restricted areas. Interestingly, F-actin depolymerization partially restored receptor mobility in cholesterol-depleted membranes. Our observations suggest that membrane cholesterol provides a dynamic environment that facilitates the free motion of EGFR and HER2, possibly by modulating the dynamic state of F-actin. The association of the receptors with lipid rafts could therefore promote their rapid interactions only upon ligand stimulation.en
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.relation.ispartofBiophysical Journalen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCholesterolen
dc.subjectHER2en
dc.subjectReceptorsen
dc.subjectEGFen
dc.subjectMembraneen
dc.titleCholesterol dictates the freedom of EGF receptors and HER2 in the plane of the membraneen_US
dc.typeArticleen_US
dc.authoridTR7497
dc.institutionauthorÖzçelik, Serdar-
dc.departmentIzmir Institute of Technology. Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1529/biophysj.104.056192-
dc.relation.doi10.1529/biophysj.104.056192en_US
dc.coverage.doi10.1529/biophysj.104.056192en_US
dc.identifier.scopusqualityQ1-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.grantfulltextopen-
item.openairetypeArticle-
item.languageiso639-1en-
crisitem.author.dept04.01. Department of Chemistry-
Appears in Collections:Chemistry / Kimya
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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