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Title: Pistacia terebinthus extract: As a potential antioxidant, antimicrobial and possible ß-glucuronidase inhibitor
Authors: Demirbüker Kavak, Dilek
Altıok, Evren
Bayraktar, Oğuz
Ülkü, Semra
Keywords: Enzyme inhibition
Plant extract
Inhibition kinetics
Pistacia terebinthus
Issue Date: Jul-2010
Publisher: Elsevier Ltd.
Source: Demirbüker Kavak, D., Altıok, E., Bayraktar, O., and Ülkü, S. (2010). Pistacia terebinthus extract: As a potential antioxidant, antimicrobial and possible β-glucuronidase inhibitor. Journal of Molecular Catalysis B: Enzymatic, 64(3-4), 167-171. doi:10.1016/j.molcatb.2010.01.029
Abstract: In this study, inhibition of Escherichia coli glucuronidase (GUS) by a plant extract of Pistacia terebinthus was investigated. P. terebinthus (from Karaburun region in Turkey) leaf extract exhibits various bioactivities, such as antioxidant, antimicrobial and cytotoxic property because of its flavonoid, phenolic and alkaloid contents. HPLC analyses were performed to evaluate the composition of the extract and main flavonoid compounds such as luteolin, luteolin-7-glucoside and apigenin-7-glucoside were detected in extract. GUS activity and kinetic data of the GUS were obtained by measuring rate of production of resulting fluorescent 4-methylumbelliferone (4-MU) and phenolphthalein fluorometrically and spectrophotometrically, respectively. Plant leaves showed higher antioxidant capacity as 85 TEAC (trolox equivalent antioxidant capacity) value and higher antimicrobial activity towards Staphylococcus aureus. 92.4% GUS enzyme inhibition was achieved with 8.3 μg ml-1 crude extract concentration, where IC50 value was found as 2.1 (±0.2) μg ml-1. Kinetic studies by nonlinear regression revealed that inhibition type caused by plant extract was mixed type (noncompetitive and competitive together). Km and Vmax values were 96.94 ± 9.68 nM and 7.81 ± 0.16 nmol min-1 mg-1, respectively. Inhibition constant (αKi) was found as 7.00 ± 0.93 nM having α value 2.17 ± 0.93. Our results may provide useful information regarding the inhibition of microbial GUS activity, preventing deglucuronidation and reducing possible cancer risk by bioactive P. terebinthus crude extract. © 2010 Elsevier B.V. All rights reserved.
ISSN: 1381-1177
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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