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Title: Colloid deposition experiments as a diagnostic tool for biomass attachment onto bioproduct adsorbent surfaces
Authors: Tarı, Canan
Vennapusa, Rami Reddy
Cabrera, Rosa B.
Fernandez-Lahore, Marcelo
Keywords: Attachment efficiency
Biomass deposition
Direct capture
Interfacial energy
Issue Date: Feb-2008
Publisher: John Wiley and Sons Inc.
Source: Tarı, C., Vennapusa, R. R., Cabrera, R. B., and Fernandez-Lahore, M. (2008). Colloid deposition experiments as a diagnostic tool for biomass attachment onto bioproduct adsorbent surfaces. Journal of Chemical Technology and Biotechnology, 83(2), 183-191. doi:10.1002/jctb.1852
Abstract: Background: Detrimental processing conditions can be expected in any downstream operation where direct contacting between a crude feedstock and a reactive solid phase is supposed to occur. In this paper we have investigated the factors influencing intact yeast cells deposition onto anion and cation exchangers currently utilized for expanded-bed adsorption of biotechnological products. The aim of this study was twofold: (a)to confirm previous findings relating biomass deposition with surface energetics according to the extended Derjaguin, Landau, Verwey and Overbeek theory (XDLVO) theory; and (b) to provide a simple experimental tool to evaluate biomass deposition onto process surfaces. Results: Biomass deposition experiments were performed on an automated workstation utilizing a packedbed format. Two commercial ion exchangers intended for the direct capture of bioproducts in the presence of suspended biological particles were employed. Intact yeast cells in the late exponential phase of growth were selected as model bio-colloids. Cell deposition was systematically evaluated as a function of fluid-phase conductivity and quantitatively expressed as a biomass deposition parameter (α). Conclusion: α ≤ 0.15 was established as a criterion to reflect negligible biomass adhesion to the process support(s). Biomass deposition experiments further confirmed predictions made on the basis of free interfacial energy calculations as per the extended DLVO approach.
ISSN: 0268-2575
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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