Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2268
Title: Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology
Authors: Tarı, Canan
Göğüş, Nihan
Tokatlı, Figen
Tarı, Canan
Göğüş, Nihan
Tokatlı, Figen
Izmir Institute of Technology. Food Engineering
Keywords: Biomass
Aspergillus sojae
Fungal morphology
Pectinase
Response surface methodology
Issue Date: Apr-2007
Publisher: Elsevier Ltd.
Source: Tarı, C., Göğüş, N., and Tokatlı, F. (2007). Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology. Enzyme and Microbial Technology, 40(5), 1108-1116. doi:10.1016/j.enzmictec.2006.08.016
Abstract: A two-step optimization procedure using central composite design with four factors (concentrations of maltrin and corn steep liquor (CSL), agitation speed and inoculation ratio) was used in order to investigate the effect of these parameters on the polygalacturonase (PG) enzyme activity, mycelia growth (biomass) and morphology (pellet size) of Aspergillus sojae ATCC 20235. According to the results of response surface methodology (RSM), initial concentrations of maltrin and CSL and agitation speed were significant (p < 0.05) on both PG enzyme production and biomass formation. As a result of this optimization, maximum PG activity (13.5 U/ml) was achievable at high maltrin (120 g/l), at low CSL (0 g/l), high agitation speed (350 rpm) and high inoculation ratio (2 × 107 total spore). Similarly, maximum biomass (26 g/l) could be obtained under the same conditions with only the difference for higher level of CSL requirement. The diameter of pellets in all optimization experiments ranged between 0.05 and 0.76 cm. The second optimization step improved the PG activity by 74% and the biomass by 40%.
URI: http://doi.org/10.1016/j.enzmictec.2006.08.016
http://hdl.handle.net/11147/2268
ISSN: 0141-0229
0141-0229
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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