Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2228
Title: Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L21
Authors: Tarı, Canan
Gençkal, Hande
Tokatlı, Figen
Tarı, Canan
Gençkal, Hande
Tokatlı, Figen
Izmir Institute of Technology. Food Engineering
Keywords: Enzymes
Alkaline protease
Bacillus sp.
Low cost media
Media optimization
Issue Date: Mar-2006
Publisher: Elsevier Ltd.
Source: Tarı, C., Gençkal, H., and Tokatlı, F. (2006). Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L21. Process Biochemistry, 41(3), 659-665. doi:10.1016/j.procbio.2005.08.012
Abstract: Bacillus sp. L21 was isolated from the by-products of a leather factory (located in Izmir, Turkey) working under extreme alkaline conditions. Its phenotypic and genotypic identifications were completed, and determined as a potential alkaline protease producer. After screening various elements, carbon and nitrogen sources, soybean meal, maltose50, tween80 and the initial pH conditions were chosen as main factors to be used in the experimental design and response surface methodology (RSM) for the optimization of a low cost enzyme producing media for potential use on an industrial scale. The optimized values obtained by the statistical analysis showed that soybean meal at 3.0 g/l, maltose50 between the ranges of 30 and 40 g/l, tween80 at 0.35 g/l and an initial pH of 8.0 gives maximum protease activity.
URI: http://doi.org/10.1016/j.procbio.2005.08.012
http://hdl.handle.net/11147/2228
ISSN: 1359-5113
0032-9592
1359-5113
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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