Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2111
Title: Evidence for the presence of a second electron donor for the cytoplasmic thioredoxins in the yeast Saccharomyces cerevisiae
Other Titles: Maya (Saccharomyces cerevisiae) sitoplazmik tiyoredoksinleri için ikinci bir elektron donörünün varlığını gösteren deliller
Authors: Koç, Ahmet
Karakaya, Hüseyin Çağlar
Ünlü, Ercan Selçuk
Koç, Ahmet
Karakaya, Hüseyin Çağlar
Ünlü, Ercan Selçuk
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Methionine
Methionine sulfoxide
Saccharomyces cerevisiae
Thioredoxin
Thioredoxin reductase
Yeast
Issue Date: 2006
Publisher: TUBITAK
Source: Koç, A., Karakaya, H. Ç., and Ünlü, E. S. (2006). Evidence for the presence of a second electron donor for the cytoplasmic thioredoxins in the yeast Saccharomyces cerevisiae. Turkish Journal of Biology, 30(3), 133-138.
Abstract: In yeast, the cytoplasmic thioredoxin system is composed of NADPH, thioredoxin reductase-1 (TRR1) and 2 thioredoxin genes (TRX1, TRX2). In this study, using yeast knockout mutants for TRR1, TRX1 and TRX2 genes, the role of the thioredoxin system in methionine sulfoxide reduction was investigated. Cells lacking both TRX1 and TRX2 genes simultaneously were not able to reduce methionine sulfoxides to methionine; however, mutants missing the TRR1 gene were able to reduce methionine sulfoxides to methionine, which showed that electrons could be transferred from NADPH to thioredoxins in the absence of TRR1. Similar results were observed for 3-phosphoadenosine 5-phosphosulfate reduction in the inorganic sulfate assimilation pathway. Results from both assays suggested that yeast cells have additional cytoplasmic thioredoxin reductase activity that could compensate for methionine sulfoxide reduction and sulfate assimilation in the absence of TRR1. This report also constitutes the first evidence that thioredoxins are the in vivo electron donors for methionine sulfoxide reductases in yeast.
URI: http://hdl.handle.net/11147/2111
ISSN: 1300-0152
1300-0152
1303-6092
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection

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