Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/1972
Title: Production of antimicrobial films by incorporation of partially purified lysozyme into biodegradable films of crude exopolysaccharides obtained from aureobasidium pullulans fermentation
Authors: Kandemir, Nilay
Yemenicioğlu, Ahmet
Mecitoğlu, Çiğdem
Elmacı, Zehra Seda
Arslanoğlu, Alper
Göksungur, Mehmet Yekta
Baysal, Taner
Yemenicioğlu, Ahmet
Mecitoğlu, Çiğdem
Elmacı, Zehra Seda
Arslanoğlu, Alper
Izmir Institute of Technology. Food Engineering
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Antimicrobial films
Aureobasidium pullulans
Exopolysaccharides
Lysozyme
Pullulan
Issue Date: Oct-2005
Publisher: University of Zagreb
Source: Kandemir, N., Yemenicioǧlu, A., Mecitoǧlu, Ç., Elmacı, Z.S., Arslanoǧlu, A., Göksungur, M. Y., and Baysal, T. (2005). Production of antimicrobial films by incorporation of partially purified lysozyme into biodegradable films of crude exopolysaccharides obtained from aureobasidium pullulans fermentation. Food Technology and Biotechnology, 43(4), 343-350.
Abstract: Antimicrobial films were produced by incorporating partially purified lysozyme into films of crude exopolysaccharides (59% pullulan) obtained from Aureobasidium pullulans fermentation. After film making, the films containing lysozyme at 100, 260, 520 and 780 μg/cm2 showed 23 to 70% of their expected enzyme activities. The highest recovery of enzyme activity (65-70%) after the film making was obtained in films prepared by incorporating lysozyme at 260 μg/cm2 (1409 U/cm2). The incorporation of disodium EDTA·2H2O and sucrose did not affect the initial lysozyme activity of the films significantly. With or without the presence of disodium EDTA·2H2O at 52 or 520 μg/cm2, lysozyme activity showed sufficient stability in the films during 21 days of cold storage. However, the presence of sucrose at 10 mg/cm2 in the films caused the destabilization of part of enzyme activity (almost 35%) at the end of storage. The combinational incorporation of lysozyme at 780 μg/cm 2 (4227 U/cm2) and disodium EDTA·2H2O at 520 μg/cm2 gave antimicrobial films effective on Escherichia coli. However, in the studied lysozyme concentration range the films did not show any antimicrobial activity against Lactobacillus plantarum. This study clearly showed that the partially purified lysozyme and crude exopolysaccharides from Aureobasidium pullulans may be used to obtain antimicrobial films to increase the safety of foods.
URI: http://hdl.handle.net/11147/1972
ISSN: 1330-9862
1330-9862
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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