Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/15603
Title: An Ex Vivo Model for Evaluation of Prebiotic Activity of Xylan and Xylooligosaccharides
Authors: Sabanci, Kevser
Gulec, Sukru
Buyukkileci, Ali Oguz
Keywords: Ex Vivo Model
Prebiotics
Xylan
Xylooligosaccharides
Large Intestine
Publisher: Elsevier
Abstract: Ex vivo techniques can provide more physiologically significant insights into prebiotic activity and overcome some limitations of in vitro tests. In this study, an ex vivo model, formed of a large intestine of mice, was tested to assess the effects of the hydrocolloidal natural polymer, xylan (XY), and its hydrolysis product, xylooligosaccharides (XOS). XY and XOS were loaded separately into the cecum, proximal colon, and distal colon. Their utilization and short-chain fatty acid (SCFA) formation by the colonized microflora and levels of dominant phyla and key genera such as Bifidobacterium, Bacteroides, and Lactobacillus were followed. XY and XOS were metabolized in all sections, and SCFAs were released. The results suggest that the slower utilization of XY compared to XOS in the cecum can enable this polysaccharide to move towards distal parts of the large intestine and extend the sites of prebiotic activity. Unlike widely used in vitro models, the ex vivo model allowed testing the utilization pattern and effects of the prebiotics in the natural environment of the microflora and examining the intestinal sections separately.
URI: https://doi.org/10.1016/j.foodres.2025.116461
ISSN: 0963-9969
1873-7145
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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