Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/14867
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dc.contributor.authorKirlangic, Irem Aydin-
dc.contributor.authorUner, Goklem-
dc.contributor.authorKara, Pinar-
dc.contributor.authorKirmizibayrak, Petek Ballar-
dc.contributor.authorErtas, Fatma Nil-
dc.date.accessioned2024-10-25T23:18:52Z-
dc.date.available2024-10-25T23:18:52Z-
dc.date.issued2024-
dc.identifier.issn1618-2642-
dc.identifier.issn1618-2650-
dc.identifier.urihttps://doi.org/10.1007/s00216-024-05529-w-
dc.identifier.urihttps://hdl.handle.net/11147/14867-
dc.description.abstractIn this study, a novel aptasensor based on a transition metal oxide-modified pencil graphite electrode (PGE) was developed for the diagnosis of early-stage prostate cancer (PCa) via monitoring the prostate-specific antigen (PSA), which is the main biomarker for PCa. Single-use PGEs modified with pulsed deposited manganese oxide (MnOx) film were used to attach the amino-terminated aptamer specific to the PSA via carbodiimide chemistry. The designed aptasensor was placed in an electrochemical cell containing ferri/ferrocyanide ions as a redox probe to measure the charge transfer resistances (Rct) of the electrode surface by electrochemical impedance spectroscopy (EIS) to follow the response of each modification step. The effect of the medium pH on the ionic structure of the aptamer molecule according to its pI value and, thus, the reversing of the direction of the response (Delta Rct) by the pH change was also discussed. The level of PSA secreted from PCa cells was investigated using impedimetric transduction. The specificity of the aptasensor was validated through selectivity studies against non-specific tumor markers like VEGF and different cancer cell lines including breast cancer and androgen-insensitive prostate cancer. The developed system showcases a label-free, fast, specific, and cost-effective approach for PSA detection, highlighting the importance of medium pH and the electrostatic environment on the aptamer's response. Our work emphasizes the potential for such aptasensors in clinical diagnostics and paves the way for further exploration into using transition metal oxides in biosensing applications.en_US
dc.description.sponsorshipEge University Scientific Research Projects Coordination Unit [FDK-2020-21648]en_US
dc.description.sponsorshipThis study is a part of the PhD thesis of Irem Aydin Kirlangic supported by Ege University Scientific Research Projects Coordination Unit [FDK-2020-21648]; resources: Pinar Kara and Petek Ballar Kirmizibayrak; supervision: Pinar Kara and Fatma Nil Erta & scedil;.en_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPSAen_US
dc.subjectAptasensoren_US
dc.subjectManganese oxideen_US
dc.subjectEISen_US
dc.titleDevelopment of transition metal oxide platforms for aptasensing of PSA in cell culturesen_US
dc.typeArticleen_US
dc.departmentIzmir Institute of Technologyen_US
dc.identifier.wosWOS:001326688800002-
dc.identifier.scopus2-s2.0-85205428290-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s00216-024-05529-w-
dc.identifier.pmid39352470-
dc.authorscopusid57224411812-
dc.authorscopusid57219944322-
dc.authorscopusid6701651517-
dc.authorscopusid57222366085-
dc.authorscopusid6603057967-
dc.authorwosidErtas, Fatma/B-2699-2018-
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
dc.description.woscitationindexScience Citation Index Expanded-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.openairetypeArticle-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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