Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/14431
Full metadata record
DC FieldValueLanguage
dc.contributor.authorÖztürk,M.-
dc.contributor.authorSalih,B.-
dc.contributor.authorEroğlu,A.E.-
dc.contributor.authorBoyaci,E.-
dc.date.accessioned2024-05-05T14:59:54Z-
dc.date.available2024-05-05T14:59:54Z-
dc.date.issued2024-
dc.identifier.issn0731-7085-
dc.identifier.urihttps://doi.org/10.1016/j.jpba.2024.116074-
dc.identifier.urihttps://hdl.handle.net/11147/14431-
dc.description.abstractRapid diagnosis of diseases is one of the challenging areas in clinical research. From the analytical chemist's perspective, the main challenges are isolating the compounds from the bio-specimen and lengthy analysis times. In this regard, solid phase microextraction offers a platform to address the abovementioned challenges. Moreover, its sharp tip-thin film geometry, known as coated blade spray (CBS), can enhance the extraction and act as an ionization source in direct mass spectrometric analysis. In this study, a new CBS device specifically designed for polar analytes was prepared and optimized to determine urinary metabolites. For this purpose, polyacrylonitrile (PAN) was selected as a base polymer as it can be electrospun to form a nanofibrous structure, and it can be modified with weak ion exchange moieties to interact with polar analytes. Following the electrospinning of PAN, hydrolysis was optimized, and conditions leading to sufficient extraction enhancement without dissolving the polymer were obtained when probes were treated with 5.0 M of NaOH for 2.5 h. Using the coated blades prepared as explained, the evaluation of various extraction conditions showed that 5 min is sufficient for equilibrium extraction. In addition, the solution's ionic strength and pH significantly affect the extraction. Optimum sorption was obtained at no salt added and pH 7.0 conditions. The CBS-MS optimization showed that 10.0 µL of ACN/MeOH/H2O (40:40:20, v/v/v) with formic acid kept for 15 seconds on the blade before voltage application leads to the highest signal. The limits of quantification of the analytes are between 50 and 100 ng/mL. © 2024 Elsevier B.V.en_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (119Z863); Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAKen_US
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysisen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiomarkersen_US
dc.subjectElectrospinningen_US
dc.subjectMass spectrometryen_US
dc.subjectPolar metabolitesen_US
dc.subjectSolid phase microextractionen_US
dc.titleDevelopment and functionalization of electrospun fiber coated thin film microextraction devices for rapid mass spectrometric determination of biologically important polar moleculesen_US
dc.typeReviewen_US
dc.departmentIzmir Institute of Technologyen_US
dc.identifier.volume243en_US
dc.identifier.wosWOS:001218662000001-
dc.identifier.scopus2-s2.0-85186347177-
dc.relation.publicationcategoryDiğeren_US
dc.identifier.doi10.1016/j.jpba.2024.116074-
dc.identifier.pmidPubMed:38437785-
dc.authorscopusid58912730800-
dc.authorscopusid7005901994-
dc.authorscopusid7005840347-
dc.authorscopusid35739711400-
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityN/A-
item.grantfulltextnone-
item.openairetypeReview-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Show simple item record



CORE Recommender

Page view(s)

48
checked on Jul 15, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.