Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/14173
Title: Enzymatic hydrolysis of organosolv-pretreated corncob and succinic acid production by Actinobacillus succinogenes
Authors: Buyukoztekin, Gulperi Karanfil
Buyukkileci, Ali Oguz
Keywords: Corncob
Organosolv
Enzymatic hydrolysis
Succinic acid
Saccharification
Publisher: Elsevier
Abstract: In this study, the conversion of organosolv-treated corncob into monosaccharides through enzymatic sacchari-fication was investigated, with the resulting monosaccharides being utilized as a carbon source to produce succinic acid. The synergy between the cellulase and xylanase provided 76% cellulose and 64% xylan di-gestibility at 50 degrees C and pH 5.2. In separate hydrolysis and fermentation (SHF), Actinobacillus succinogenes pro-duced 12.7 g/L of succinic acid from the hydrolysate with 0.12 g/g yield based on the pretreated corncob. Simultaneous saccharification and fermentation (SSF) demonstrated better performance with 16 g/L succinic acid titer and 0.24 g/g yield, though SHF provided a higher production rate. The condition in the SSF (37 degrees C and pH near neutral) was suboptimal for the enzymes, thus the succinic acid production was limited by the saccharification step. These findings emphasize the potential of organosolv-treated corncob to serve as an enzymatic hydrolysis substrate without neutralization and detoxification, supplying glucose and xylose for succinic acid production by A. succinogenes.
Description: Buyukkileci, Ali Oguz/0000-0002-0784-0008
URI: https://doi.org/10.1016/j.indcrop.2023.117922
ISSN: 0926-6690
1872-633X
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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