Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/14146
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dc.contributor.authorDikici, Serkan-
dc.date.accessioned2024-01-06T07:21:33Z-
dc.date.available2024-01-06T07:21:33Z-
dc.date.issued2023-
dc.identifier.issn2147-1762-
dc.identifier.urihttps://doi.org/10.35378/gujs.1040277-
dc.identifier.urihttps://hdl.handle.net/11147/14146-
dc.description.abstractTissue engineering (TE) enables the development of functional synthetic substitutes to be replaced with damaged tissues and organs instead of the use of auto or allografts. A wide range of biomaterials is currently in use as TE scaffolds. Among these materials, naturally sourced ones are favorable due to being highly biocompatible and supporting cell growth and function, whereas synthetic ones are advantageous because of the high tunability on mechanical and physical properties as well as being easy to process. Alongside the advantages of synthetic polymers, they mostly show hydrophobic behavior that limits biomaterial-cell interaction and, consequently, the functioning of the developed TE constructs. In this study, we assessed the impact of L-Ascorbic acid 2-phosphate (AA2P) on improving the culture conditions of human dermal fibroblasts (HDFs) growing on a three-dimensional (3D) scaffold made of polycaprolactone (PCL) using emulsion templating. Our results demonstrated that AA2P enhances the metabolic activity and growth of HDFs as well as collagen deposition by them when supplemented in their growth medium at 50 µg/mL concentration. It showed a great potential to be used as a growth medium supplement to circumvent the disadvantages of culturing human cells on a synthetic biomaterial that is not favored in default. AA2P's potential to improve cell growth and collagen deposition may prove an effective way to culture human cells on 3D PCL PolyHIPE scaffolds for various TE applications. © 2023, Gazi Universitesi. All rights reserved.en_US
dc.description.sponsorshipİzmir Yüksek Teknoloji Enstitüsü; Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK: 221S594; Bergens Forskningsstiftelse: 2021IYTE-1-0057en_US
dc.language.isoenen_US
dc.publisherGazi Üniversitesien_US
dc.relation.ispartofGazi University Journal of Scienceen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject3D cultureen_US
dc.subjectEmulsion templatingen_US
dc.subjectFibroblastsen_US
dc.subjectHuman dermalen_US
dc.subjectL-ascorbic aciden_US
dc.subjectPolycaprolactoneen_US
dc.titleAscorbic acid enhances the metabolic activity, growth and collagen production of human dermal fibroblasts growing in three-dimensional (3D) cultureen_US
dc.typeArticleen_US
dc.departmentİzmir Institute of Technology. Bioengineeringen_US
dc.identifier.volume36en_US
dc.identifier.issue4en_US
dc.identifier.startpage1625en_US
dc.identifier.endpage1637en_US
dc.identifier.wosWOS:001122774900001en_US
dc.identifier.scopus2-s2.0-85162920440en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.35378/gujs.1040277-
dc.authorscopusid57188879686-
item.languageiso639-1en-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.openairetypeArticle-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Bioengineering / Biyomühendislik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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