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https://hdl.handle.net/11147/14121
Title: | Investigating Influences of Intravenous Fluids on Huvec and U937 Monocyte Cell Lines Using the Magnetic Levitation Method | Authors: | Keçili, Seren Kaymaz, Sumeyra Vural Özoğul, Beyzanur Tekin, H. Cumhur Elitaş, Meltem |
Publisher: | ROYAL SOC CHEMISTRY | Abstract: | Intravenous fluids are being widely used in patients of all ages for preventing or treating dehydration in the intensive care units, surgeries in the operation rooms, or administering chemotherapeutic drugs at hospitals. Dextrose, Ringer, and NaCl solutions are widely received as intravenous fluids by hospitalized patients. Despite their widespread administration for over 100 years, studies on their influences on different cell types have been very limited. Increasing evidence suggests that treatment outcomes might be altered by the choice of the administered intravenous fluids. In this study, we investigated the influences of intravenous fluids on human endothelial (HUVEC) and monocyte (U937) cell lines using the magnetic levitation technique. Our magnetic levitation platform provides label-free manipulation of single cells without altering their phenotypic or genetic properties. It allows for monitoring and quantifying behavior of single cells by measuring their levitation heights, deformation indices, and areas. Our results indicate that HUVEC and U937 cell lines respond differently to different intravenous fluids. Dextrose solution decreased the viability of both cell lines while increasing the heterogeneity of areas, deformation, and levitation heights of HUVEC cells. We strongly believe that improved outcomes can be achieved when the influences of intravenous fluids on different cell types are revealed using robust, label-free, and efficient methods. Label-free analysis of cells exposed to intravenous fluids can be achieved through magnetic levitation technology coupled with cell-morphology characterization. | URI: | https://doi.org/10.1039/d3an01304a https://hdl.handle.net/11147/14121 |
ISSN: | 0003-2654 1364-5528 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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