Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/14047
Title: Enhanced production of 3-phenyllactic acid from novel non-axenic coculture: adaptive evolution and statistical fermentation studies
Authors: Meruvu, Haritha
Keywords: Antimicrobial activity
Cheese whey
Coculture
Fermentation
Lactic acid bacteria
Phenyllactic acid
Issue Date: 2023
Publisher: Springer
Abstract: This research pivots around screening of idoneous lactic acid bacteria (LAB) from cow milk and subjecting them to adaptive evolution experiments to aid superior growth/robustness necessary for 3-phenyllactic acid (3-PLA) production. Conventional and statistical fermentation studies were conducted at batch scale using a non-axenic coculture of three novel LAB strains: Lactiplantibacillus plantarum str. nov. plantharim, Lactobacillus delbrueckki str. nov. delharim, and Pediococcus pentasaceous str. nov. pentharim. Statistically optimized fermentation using Box Behnken technique resulted in 1225 mg/L 3-PLA production using the growth medium: cheese whey—MRS medium mixture (5:2 ratio), phenylalanine (2.69% w/v), and glucose (9.6% w/v). Statistical optimization of fermentation parameters resulted in a substantial increase (17 times higher) compared to the non-optimized fermentation conditions (72 mg/L). Monad growth kinetics of the cow milk whey (CMW) coculture were calculated and estimated as: μmax = 0.336 h−1, K s = 11.64 mg/mL, Yx/s = 0.835 mg/g, Y P/S = 1.66 mg/g, Y X/P = 0.112 mg/mg. The purified 3-PLA (1.93 mg/mL) showed antimicrobial activity with pathogenic bacteria like Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, with a minimum inhibitory concentration of 12 mg/mL. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
URI: https://doi.org/10.1007/s13399-023-04895-2
https://hdl.handle.net/11147/14047
ISSN: 2190-6815
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Files in This Item:
File SizeFormat 
Enhanced-production.pdf3.6 MBAdobe PDFView/Open
Show full item record



CORE Recommender

Page view(s)

22
checked on Mar 4, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.